Product nameAnti-TLR9 antibody
See all TLR9 primary antibodies
DescriptionRabbit polyclonal to TLR9
Tested applicationsSuitable for: ICC, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to 15 amino acids from an internal sequence of human TLR9.
- Jurkat cells, Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab52967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 2 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 115 kDa.|
FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
- Information by UniProt
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
Lane 1 : Anti-TLR9 antibody (ab52967) at 0.5 µg/ml
Lane 2 : Anti-TLR9 antibody (ab52967) at 1 µg/ml
Lane 3 : Anti-TLR9 antibody (ab52967) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 115 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Fixed with 4%-PFA. Immunocytochemical analysis of Jurkat cells using ab52967 at 2 µg/ml.
This product has been referenced in:
- Eichholz K et al. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells. PLoS Pathog 12:e1005871 (2016). WB ; Human . Read more (PubMed: 27636895) »
- Vegna S et al. NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase. J Virol 90:6022-35 (2016). WB . Read more (PubMed: 27099311) »