Overview

  • Product name

  • Description

    Rabbit polyclonal to TLR9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the center of human TLR9

  • Positive control

    • Jurkat cells and cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 115 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity

    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Images

  • Lane 1 : Anti-TLR9 antibody (ab62577) at 0.5 µg/ml
    Lane 2 : Anti-TLR9 antibody (ab62577) at 1 µg/ml
    Lane 3 : Anti-TLR9 antibody (ab62577) at 2 µg/ml

    All lanes : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 115 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?

  • ab62577 at 2µg/ml staining TLR9 in Jurkat cells by Immunocytochemistry.
  • Immunofluorescence of TLR9 in Mouse Spleen cells using ab62577 at 10 ug/ml.

References

This product has been referenced in:

  • Tripathi A  et al. Heteromerization of chemokine (C-X-C motif) receptor 4 with a1A/B-adrenergic receptors controls a1-adrenergic receptor function. Proc Natl Acad Sci U S A 112:E1659-68 (2015). Read more (PubMed: 25775528) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab62577.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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