Key features and details
- Rabbit polyclonal to TLR9
- Suitable for: ICC, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TLR9 antibody
See all TLR9 primary antibodies
DescriptionRabbit polyclonal to TLR9
Tested applicationsSuitable for: ICC, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 15 amino acids near the center of human TLR9
- Jurkat cells and cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 2 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 115 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
- Information by UniProt
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
Lane 1 : Anti-TLR9 antibody (ab62577) at 0.5 µg/ml
Lane 2 : Anti-TLR9 antibody (ab62577) at 1 µg/ml
Lane 3 : Anti-TLR9 antibody (ab62577) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 115 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
ab62577 at 2µg/ml staining TLR9 in Jurkat cells by Immunocytochemistry.
Immunofluorescence of TLR9 in Mouse Spleen cells using ab62577 at 10 ug/ml.
ab62577 has been referenced in 1 publication.
- Tripathi A et al. Heteromerization of chemokine (C-X-C motif) receptor 4 with a1A/B-adrenergic receptors controls a1-adrenergic receptor function. Proc Natl Acad Sci U S A 112:E1659-68 (2015). PubMed: 25775528