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Anti-TLR9 antibody - Aminoterminal end (ab53396)

Submit a review Q&A (1)References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)

Key features and details

  • Goat polyclonal to TLR9 - Aminoterminal end
  • Suitable for: IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-TLR9 antibody - Aminoterminal end
    See all TLR9 primary antibodies

  • Description

    Goat polyclonal to TLR9 - Aminoterminal end

  • Host species

    Goat

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee

  • Immunogen

    31 amino acid (aa) synthetic peptide

    SLKYNNLTVVPRNLPSSLEYLLLSYNRIVKL

    corresponding to aa 205-235 of the N-terminal domain of Human TLR9
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • Tissue specificity

    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • Cellular localization

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 289 antibody
      • CD289 antibody
      • TLR 9 antibody
      • TLR9 antibody
      • TLR9_HUMAN antibody
      • Toll like receptor 9 antibody
      • Toll like receptor 9 isoform A precursor antibody
      • Toll like receptor 9 isoform B antibody
      • Toll-like receptor 9 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      IH on paraffin section of human spleen using ab53396 at a concentration of 1/250.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      IH on paraffin section of human tonsil using ab53396 at a concentration of 1/250.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody - Aminoterminal end (ab53396)
      IH on paraffin section of mouse spleen using ab53396 at a concentration of 1/250.

    References (6)

    Publishing research using ab53396? Please let us know so that we can cite the reference in this datasheet.

    ab53396 has been referenced in 6 publications.

    • Zheng H  et al. Depletion of Toll-Like Receptor-9 Attenuates Renal Tubulointerstitial Fibrosis After Ischemia-Reperfusion Injury. Front Cell Dev Biol 9:641527 (2021). PubMed: 33644078
    • Zheng H  et al. Hydroxychloroquine Inhibits Macrophage Activation and Attenuates Renal Fibrosis After Ischemia-Reperfusion Injury. Front Immunol 12:645100 (2021). PubMed: 33936063
    • Lai JH  et al. Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways. iScience 24:102498 (2021). PubMed: 34142025
    • O'Sullivan KM  et al. Intrarenal Toll-like receptor 4 and Toll-like receptor 2 expression correlates with injury in antineutrophil cytoplasmic antibody-associated vasculitis. Am J Physiol Renal Physiol 315:F1283-F1294 (2018). PubMed: 29923769
    • Lai JH  et al. Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells. EMBO Rep 19:N/A (2018). PubMed: 29880709
    • Thiyagarajan D  et al. Silencing of renal DNaseI in murine lupus nephritis imposes exposure of large chromatin fragments and activation of Toll like receptors and the Clec4e. PLoS One 7:e34080 (2012). IHC-P, IHC-Fr ; Human, Mouse . PubMed: 22479529

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Lot number: GR4591-1
    Inquiry: Hello, we tested this antibody (1/250) for TLR9 detection on FFPE tissue sections under different antigen retrieval conditions : 10mM citrate buffer pH6, EDTA pH8.8, 1% trypsin and pepsin (ready-to-use commercial solution). The secondary antibody is a donkey anti-goat IgG - HRP and we used the Tyramide amplification system. All conditions without success. Could you give us some indications to become successful with this antibody ? Thank you for your answer Best regards

    Read More
    Answer

    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

    Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

    Read More

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