Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14964-2] to TLR9
- Suitable for: WB
- Reacts with: Human
Product nameAnti-TLR9 antibody [EPR14964-2]
See all TLR9 primary antibodies
DescriptionRabbit monoclonal [EPR14964-2] to TLR9
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human TLR9 aa 400-550 (internal sequence). The exact sequence is proprietary.
Database link: Q9NR96
- Daudi, Raji and Ramos cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab187148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa).|
FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
- Information by UniProt
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
All lanes : Anti-TLR9 antibody [EPR14964-2] (ab187148) at 1/5000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Blocking and dilution buffer: 5% NFDM/TBST
ab187148 has not yet been referenced specifically in any publications.