Overview

  • Product name

    Anti-TLR9 antibody [EPR21735]
    See all TLR9 primary antibodies
  • Description

    Rabbit monoclonal [EPR21735] to TLR9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TLR9 aa 1-350. The exact sequence is proprietary.
    Database link: Q9NR96

  • Positive control

    • WB: Raji and Daudi whole cell lysates. IP: Raji whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 120, 60 kDa (predicted molecular weight: 116 kDa).
IP 1/30.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity

    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Images

  • All lanes : Anti-TLR9 antibody [EPR21735] (ab211012) at 1/1000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
    Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 116 kDa
    Observed band size: 120,60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

    Untreated THP1s have undetectable TLR9, as described in the literature (PMID: 20375564).

  • Anti-TLR9 antibody [EPR21735] (ab211012) at 1/1000 dilution + Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 116 kDa
    Observed band size: 120,60 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

  • TLR9 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab211012 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211012 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Raji whole cell lysate 10 µg (Input). 

    Lane 2: ab211012 IP in Raji whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211012 in Raji whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

References

ab211012 has not yet been referenced specifically in any publications.

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