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RecombinantRabMAb

Recombinant Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)

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Immunoprecipitation - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
  • Western blot - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
  • Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21735] to TLR9 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-TLR9 antibody [EPR21735] - BSA and Azide free
    See all TLR9 primary antibodies

  • Description

    Rabbit monoclonal [EPR21735] to TLR9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji whole cell lysate.

  • General notes

    ab232933 is the carrier-free version of ab211012.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab232933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 120, 60 kDa (predicted molecular weight: 116 kDa).
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 120, 60 kDa (predicted molecular weight: 116 kDa).
IP
Use at an assay dependent concentration.

Target

  • Function

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • Tissue specificity

    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • Cellular localization

    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 289 antibody
      • CD289 antibody
      • TLR 9 antibody
      • TLR9 antibody
      • TLR9_HUMAN antibody
      • Toll like receptor 9 antibody
      • Toll like receptor 9 isoform A precursor antibody
      • Toll like receptor 9 isoform B antibody
      • Toll-like receptor 9 antibody
      see all

    Images

    • Immunoprecipitation - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
      Immunoprecipitation - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)

      TLR9 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab211012 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211012 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Raji whole cell lysate 10 µg (Input). 

      Lane 2: ab211012 IP in Raji whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211012 in Raji whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

      The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211012).

    • Western blot - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
      Western blot - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
      All lanes : Anti-TLR9 antibody [EPR21735] (ab211012) at 1/1000 dilution

      Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
      Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 116 kDa
      Observed band size: 120,60 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).

      Untreated THP1s have undetectable TLR9, as described in the literature (PMID: 20375564).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211012).

    • Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
      Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab232933? Please let us know so that we can cite the reference in this datasheet.

    ab232933 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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