Recombinant Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)
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Overview
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Product name
Anti-TLR9 antibody [EPR21735] - BSA and Azide free
See all TLR9 primary antibodies -
Description
Rabbit monoclonal [EPR21735] to TLR9 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human TLR9 aa 1-350. The exact sequence is proprietary.
Database link: Q9NR96 -
Positive control
- WB: Raji whole cell lysate.
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General notes
Ab232933 is the carrier-free version of ab211012. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232933 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21735 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab232933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 120, 60 kDa (predicted molecular weight: 116 kDa). | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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Database links
- Entrez Gene: 54106 Human
- Omim: 605474 Human
- SwissProt: Q9NR96 Human
- Unigene: 87968 Human
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Alternative names
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
Images
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![Immunoprecipitation - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)](https://www.abcam.com/ps/products/232/ab232933/Images/ab232933-321172-anti-tlr9-antibody-epr21735-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)TLR9 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab211012 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211012 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Raji whole cell lysate 10 µg (Input).
Lane 2: ab211012 IP in Raji whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211012 in Raji whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211012).
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![Western blot - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)](https://www.abcam.com/ps/products/232/ab232933/Images/ab232933-304261-anti-tlr9-antibody-epr21735-western-blot.jpg)
Western blot - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (ab232933)All lanes : Anti-TLR9 antibody [EPR21735] (ab211012) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 116 kDa
Observed band size: 120,60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The cleaved TLR9 (60 kDa) observed is consistent with the literature (PMID: 24582318).
Untreated THP1s have undetectable TLR9, as described in the literature (PMID: 20375564).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211012).
Datasheets and documents
References
ab232933 has not yet been referenced specifically in any publications.
