Key features and details
- Rabbit polyclonal to TLR9 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TLR9 antibody - N-terminal
See all TLR9 primary antibodies
DescriptionRabbit polyclonal to TLR9 - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Synthetic peptide within Human TLR9 (N terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: Q9NR96
- IHC-P: Human lung cancer tissue. WB: THP-1 whole cell lysate; TLR9 transfected HEK-293T lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 116 kDa.|
FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificityHighly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Sequence similaritiesBelongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Cellular localizationEndoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
- Information by UniProt
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
Paraffin-embedded human lung cancer tissue stained for TLR9 using ab227004 at 1/250 dilution in immunohistochemical analysis.
Anti-TLR9 antibody - N-terminal (ab227004) at 1/1000 dilution + THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 116 kDa
7.5% SDS PAGE gel.
All lanes : Anti-TLR9 antibody - N-terminal (ab227004) at 1/2000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : TLR9 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 116 kDa
7.5% SDS PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227004 has not yet been referenced specifically in any publications.