Product nameAnti-TLS/FUS antibody
See all TLS/FUS primary antibodies
DescriptionRabbit polyclonal to TLS/FUS
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Synthetic peptide aa 500-526 (C terminal). The exact sequence is proprietary.
Database link: P35637
- WB: HeLa, HEK-293T and Jurkat cell lysate IHC-P: Human breast carcinoma and mouse teratoma tissue. IP: HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|WB||1/10000 - 1/50000. Predicted molecular weight: 53 kDa.|
FunctionBinds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
Involvement in diseaseNote=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
Sequence similaritiesBelongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
modificationsArg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- 75 kDa DNA pairing protein antibody
- 75 kDa DNA-pairing protein antibody
- ALS6 antibody
All lanes : Anti-TLS/FUS antibody (ab264119) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma (left) and mouse teratoma (right) tissue stained for TLS/FUS using ab264119 at 1/5000 (Left) and 1/1000 (Right). Detection: DAB.
TLS/FUS was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264119 at 6 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab264119 0.1 µg/ml.
Lane 1: ab264119 IP in HeLa whole cell lysate.
Lane 2: ab264119 IP in HeLa whole cell lysate.
Lane 3: Control IgG.
Different lots of ab264119 used in lane 1 and 2.
ab264119 has not yet been referenced specifically in any publications.