Recombinant Anti-TLS/FUS antibody [BLR023E] (ab243880)
![Western blot - Anti-TLS/FUS antibody [BLR023E] (ab243880)](https://www.abcam.com/ps/products/243/ab243880/Images/ab243880-9-anti-fus-antibody-blr023e-western-blot-wildtype-hela-knockout-fus-human.jpg "Western blot - Anti-TLS/FUS antibody [BLR023E] (ab243880)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR023E] to TLS/FUS
- Suitable for: ICC/IF, IHC-P, IP, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TLS/FUS antibody [BLR023E]
See all TLS/FUS primary antibodies -
Description
Rabbit monoclonal [BLR023E] to TLS/FUS -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human TLS/FUS aa 1-50. The exact sequence is proprietary. Gene ID 2521.
Database link: P35637 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR023E -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
|
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
|
| WB |
1/1000.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/500. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
|
WB
1/1000. |
Target
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Function
Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%. -
Sequence similarities
Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsArg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2521 Human
- Entrez Gene: 233908 Mouse
- Omim: 137070 Human
- SwissProt: P35637 Human
- SwissProt: P56959 Mouse
- Unigene: 46894 Human
- Unigene: 277680 Mouse
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Alternative names
- 75 kDa DNA pairing protein antibody
- 75 kDa DNA-pairing protein antibody
- ALS6 antibody
see all
Images
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Western blot - Anti-TLS/FUS antibody [BLR023E] (ab243880)
ab243880 was shown to react with FUS in wild-type HeLa cells in Western blot with loss of signal observed in a FUS knockout cell line. Wild-type HeLa and FUS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab243880 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 1/5000 before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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![Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [BLR023E] (ab243880)](https://www.abcam.com/ps/products/243/ab243880/Images/ab243880-334312-anti-tls-fus-antibody-blr023e-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [BLR023E] (ab243880)Formaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling TLS/FUS using ab243880 at 1/250 dilution in ICC/IF analysis. A DyLight594-conjugated goat anti-rabbit IgG was used as the secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [BLR023E] (ab243880)](https://www.abcam.com/ps/products/243/ab243880/Images/ab243880-8-anti-fus-antibody-blr023e-immunocytochemistry-wildtype-hela-knockout-fus-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [BLR023E] (ab243880)ab243880 was shown to react with FUS in wild-type HeLa cells in Immunocytochemistry with loss of signal observed in a FUS knockout cell line. Wild-type and Knockout cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 5%BSA+5%goat serum (30min). The cells were then incubated with ab243880 at 1/500 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-rabbit secondary antibody to (Alexa Fluor® 555) at 0.5 μg/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (knockout) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and knockout cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880).
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] (ab243880)](https://www.abcam.com/ps/products/243/ab243880/Images/ab243880-334311-anti-tls-fus-antibody-blr023e-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] (ab243880)Formalin-fixed, paraffin-embedded human breast tissue stained for TLS/FUS using ab243880 at 1/250 dilution in immunohistochemical analysis. A DyLight594-conjugated goat anti-rabbit antibody was used as the secondary. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] (ab243880)](https://www.abcam.com/ps/products/243/ab243880/Images/ab243880-334310-anti-tls-fus-antibody-blr023e-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] (ab243880)Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for TLS/FUS using ab243880 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining. -
![Immunoprecipitation - Anti-TLS/FUS antibody [BLR023E] (ab243880)](https://www.abcam.com/ps/products/243/ab243880/Images/ab243880-334309-anti-tls-fus-antibody-blr023e-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TLS/FUS antibody [BLR023E] (ab243880)TLS/FUS was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243880 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243880 at 1/1000 dilution.Lane 1: ab243880 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
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![Western blot - Anti-TLS/FUS antibody [BLR023E] (ab243880)](https://www.abcam.com/ps/products/243/ab243880/Images/ab243880-334308-AntiTLSFUS-antibody-BLR023E-wb.jpg)
Western blot - Anti-TLS/FUS antibody [BLR023E] (ab243880)All lanes : Anti-TLS/FUS antibody [BLR023E] (ab243880) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG antibodyLower Panel: Rabbit anti-FUS recombinant monoclonal antibody.
Detection: chemiluminescence with an exposure time of 30 seconds
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![Anti-TLS/FUS antibody [BLR023E] (ab243880)](https://www.abcam.com/ps/products/243/ab243880/Images/ab243880-6-benefits-of-recombinant-antibodies.png)
Anti-TLS/FUS antibody [BLR023E] (ab243880)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243880 has not yet been referenced specifically in any publications.