Product nameAnti-TLS/FUS antibody [CL0190]
See all TLS/FUS primary antibodies
DescriptionMouse monoclonal [CL0190] to TLS/FUS
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human kidney, stomach, prostate and pancreas tissues. WB: U-251 cells. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab154141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 53 kDa.|
|ICC/IF||Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionBinds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
Involvement in diseaseNote=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
Sequence similaritiesBelongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
modificationsArg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- 75 kDa DNA pairing protein antibody
- 75 kDa DNA-pairing protein antibody
- ALS6 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TLS/FUS knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab154141 observed at 80 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab154141 was shown to specifically react with TLS/FUS when TLS/FUS knockout samples were used. Wild-type and TLS/FUS knockout samples were subjected to SDS-PAGE. ab154141 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD)preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TLS/FUS with ab154141 showing nuclear (without nucleoli) staining in green. Microtubule are visualized in red.
All lanes : Anti-TLS/FUS antibody [CL0190] (ab154141)
Lane 1 : U-251 cells, transfected with control siRNA
Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
Lane 3 : U-251 cells, transfected with target specific siRNA probe #2
Predicted band size: 53 kDa
Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunohistochemical analysis of Human kidney tissue labeling TLS/FUS with ab154141 at 1/2500 dilution.
Immunohistochemical analysis of Human prostate tissue labeling TLS/FUS with ab154141 at 1/2500 dilution.
Immunohistochemical analysis of Human stomach tissue labeling TLS/FUS with ab154141 at 1/2500 dilution.
Immunohistochemical analysis of Human pancreas tissue labeling TLS/FUS with ab154141 at 1/2500 dilution.
ab154141 has not yet been referenced specifically in any publications.