Product nameAnti-TLS/FUS antibody [EPR5812]
See all TLS/FUS primary antibodies
DescriptionRabbit monoclonal [EPR5812] to TLS/FUS
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TLS/FUS. The exact sequence is proprietary.
- K562, Human fetal brain, Caco 2, and HepG2 lysates; Human kidney tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.55 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 53 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
FunctionBinds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
Involvement in diseaseNote=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
Sequence similaritiesBelongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
modificationsArg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- 75 kDa DNA pairing protein antibody
- 75 kDa DNA-pairing protein antibody
- ALS6 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TLS/FUS knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab124923 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124923 was shown to specifically react with TLS/FUS when TLS/FUS knockout samples were used. Wild-type and TLS/FUS knockout samples were subjected to SDS-PAGE. ab124923 and ab28245 (loading control to GAPDH) were both diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-TLS/FUS antibody [EPR5812] (ab124923) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
ab124923, at 1/50 dilution, staining TLS/FUS in formalin-fixed, paraffin-embedded Human kidney tissue, by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Urdaneta EC et al. Purification of cross-linked RNA-protein complexes by phenol-toluol extraction. Nat Commun 10:990 (2019). Read more (PubMed: 30824702) »
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). Read more (PubMed: 30377371) »