Overview

  • Product name

    Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free
    See all TLS/FUS primary antibodies
  • Description

    Rabbit monoclonal [EPR5812] to TLS/FUS - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TLS/FUS. The exact sequence is proprietary.

  • Positive control

    • WB: K562, HepG2 and wild-type HAP1 cell lysate.
  • General notes

    Ab232065 is the carrier-free version of ab124923. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 73 kDa (predicted molecular weight: 53 kDa).
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Note=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
    Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
    Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
    Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
  • Sequence similarities

    Belongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 75 kDa DNA pairing protein antibody
    • 75 kDa DNA-pairing protein antibody
    • ALS6 antibody
    • Amyotrophic lateral sclerosis 6 antibody
    • fus antibody
    • FUS CHOP antibody
    • Fus like protein antibody
    • FUS_HUMAN antibody
    • FUS1 antibody
    • Fused in sarcoma antibody
    • Fusion (involved in t(12;16) in malignant liposarcoma) antibody
    • Fusion derived from t(12;16) malignant liposarcoma antibody
    • Fusion gene in myxoid liposarcoma antibody
    • Heterogeneous nuclear ribonucleoprotein P2 antibody
    • hnRNP P2 antibody
    • hnRNPP2 antibody
    • Oncogene FUS antibody
    • Oncogene TLS antibody
    • POMp75 antibody
    • RNA binding protein FUS antibody
    • RNA-binding protein FUS antibody
    • TLS antibody
    • TLS CHOP antibody
    • Translocated in liposarcoma antibody
    • Translocated in liposarcoma protein antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: TLS/FUS knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 to 4: Merged signal (red and green). Green - ab124923 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab124923 was shown to specifically react with TLS/FUS when TLS/FUS knockout samples were used. Wild-type and TLS/FUS knockout samples were subjected to SDS-PAGE. ab124923 and ab28245 (loading control to GAPDH) were both diluted at 1/1,000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124923).

  • ab124923, at 1/50 dilution, staining TLS/FUS in formalin-fixed, paraffin-embedded Human kidney tissue, by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124923).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124923).

References

ab232065 has not yet been referenced specifically in any publications.

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