Overview

  • Product name

  • Description

    Rabbit polyclonal to TLX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse TLX1 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab125503)

  • Positive control

    • This antibody gave a positive signal in Mouse Hippocampus tissue lysate.
  • General notes

     This product was previously labelled as HOX11

     

Properties

Applications

Our Abpromise guarantee covers the use of ab105496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    Controls the genesis of the spleen. Binds to the DNA sequence 5'-GGCGGTAAGTGG-3'.
  • Involvement in disease

    Note=A chromosomal aberration involving TLX1 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(10;14)(q24;q11) with TCRD.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homeo box 11 (T cell leukemia 3 associated breakpoint homologous to Drosophila Notch) antibody
    • Homeo box 11 (T cell lymphoma 3 associated breakpoint) antibody
    • Homeobox 11 antibody
    • Homeobox protein Hox 11 antibody
    • Homeobox protein Hox-11 antibody
    • Homeobox TLX-1 antibody
    • Hox-11 antibody
    • Hox11 antibody
    • MGC163402 antibody
    • OTTHUMP00000020816 antibody
    • Proto oncogene TCL 3 antibody
    • Proto-oncogene TCL-3 antibody
    • RGD1563655 antibody
    • T cell leukemia homeobox protein 1 antibody
    • T cell leukemia lymphoma protein 3 antibody
    • T-all, included antibody
    • T-cell acute lymphocytic leukemia, included antibody
    • T-cell leukemia 3 gene antibody
    • T-cell leukemia homeobox protein 1 antibody
    • T-cell leukemia, homeobox 1 antibody
    • T-cell leukemia/lymphoma protein 3 antibody
    • TCL 3 proto oncogene antibody
    • TCL3 antibody
    • Tlx-1 antibody
    • TLX1 antibody
    • TLX1_HUMAN antibody
    see all

Images

  • Anti-TLX1 antibody (ab105496) at 1 µg/ml + Mouse Hippocampus Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa
    Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

References

ab105496 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Hippocampus)
Specification
Hippocampus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Mar 19 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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