Anti-TM14A antibody (ab236904)
Key features and details
- Rabbit polyclonal to TM14A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TM14A antibody -
Description
Rabbit polyclonal to TM14A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Guinea pig, Cow
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Immunogen
Recombinant fragment corresponding to Human TM14A aa 45-78.
Sequence:RVSNDKRDVKVSLFTAFFLATIMGVRFKRSKKIM
Database link: Q9Y6G1 -
Positive control
- WB: PC-3 whole cell lysate. ICC/IF: PC-3 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab236904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 11 kDa. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Sequence similarities
Belongs to the UPF0136 (TMEM14) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 28978 Human
- SwissProt: Q9Y6G1 Human
- Unigene: 94896 Human
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Alternative names
- TM14A_HUMAN antibody
- TMEM14A antibody
- Transmembrane protein 14A antibody
Images
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Western blot - Anti-TM14A antibody (ab236904)Anti-TM14A antibody (ab236904) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 11 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TM14A antibody (ab236904)PC-3 (human prostate adenocarcinoma cell line) cells stained for TM14A (green) using ab236904 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab236904 has not yet been referenced specifically in any publications.
