Product nameTMB ELISA Substrate (Highest Sensitivity)
See all ELISA substrate reagents
Tested applicationsSuitable for: ELISAmore details
Unsuitable for: IHC-Fr or IHC-P
Abcam’s TMB ELISA Substrate (Highest Sensitive) detects horseradish peroxidase (HRP) activity and contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution. Unreacted substrate should be colorless or very light yellow in appearance. When this substrate system is reacted with peroxidase, a soluble blue reaction product is obtained. The reaction can be stopped using appropriate stop solution, producing a soluble yellow or soluble blue reaction product, depending upon the stop reagent used, which is stable for at least 1 hour. ab171522 is not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.
ab171522 is supplied as a ready to use solution. The product should be allowed to equilibrate to room temperature (25°C) prior to use. For microwell applications, 100 µL of substrate solution is added to each well. A soluble blue reaction product develops which can be read at 370 nm or in a range of 620 nm to 650 nm. For best results, sample absorbance values should be monitored and read before absorbance values exceed 2.0 OD units. In endpoint assays, the substrate reaction can be stopped using equal volumes of 1 N HCl, 0.6 N sulfuric acid, or one of the stop solutions (ab171529 and ab171531). Addition of acid turns the blue color to yellow and stops the enzymatic reaction. In the case of the 650 nm Stop Solutions for TMB Substrate (ab171531), the blue color does not change. Since stopping the reaction increases sample absorbance values approximately three fold, unless using the 650 nm Stop Solutions for TMB Substrate (ab171531), sample OD values should be monitored and substrate reaction stopped when values reach approximately 0.7 OD units. After stopping with the 450 nm Stop Solutions for TMB Substrate (ab171529), a soluble yellow product develops which is read in the 450 nm range. Stopping with the 650 nm Stop Solutions for TMB Substrate (ab171531) produces a soluble blue product which is read in the 650 nm range. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.
Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule.
Storage instructionsStore at +4°C. Please see notes section.
Storage bufferpH: 1
Constituents: 0.05% TMB, 79% Water, 0.1% Hydrogen peroxide
Concentration information loading...
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Phosphate Buffered Saline (PBS) Casein ELISA Reagent (ab171532)
- Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- AP Stability Conjugate Reagent (ab171536)
- HRP Stability Conjugate Reagent (ab171537)
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab171522 has been referenced in 5 publications.
- Li X et al. The novel llama-human chimeric antibody has potent effect in lowering LDL-c levels in hPCSK9 transgenic rats. Clin Transl Med 9:16 (2020). PubMed: 32056048
- Chernyavtseva A et al. Differential recognition of peptides within feline coronavirus polyprotein 1 ab by sera from healthy cats and cats with feline infectious peritonitis. Virology 532:88-96 (2019). PubMed: 31048107
- Bailey JK et al. Synthesis of multivalent glycopeptide conjugates that mimic an HIV epitope. Tetrahedron 72:6091-6098 (2016). PubMed: 28190897
- Mathesz A et al. [Integrated optical sensor for rapid analysis of biological samples]. Orv Hetil 156:2116-9 (2015). PubMed: 26686748
- Hersey JS et al. Functionalized Nanofiber Meshes Enhance Immunosorbent Assays. Anal Chem 87:11863-70 (2015). PubMed: 26551162