Overview

  • Product name

    TMB, high kinetics, Peroxidase substrate
  • Description

    Peroxidase substrate
  • Biological description

    Peroxidase substrate. Oxidized by prostagladin synthase. Non-mutagenic chromogen used in enzyme-immunoassay. Active in vitro.
  • General notes

    This material is extremely light sensitive. Take all appropriate steps to minimise exposure to direct sunlight and all UV light sources, including sunlight. Discard if solution is blue or turbid.

  • CAS Number

    54827-17-7
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl
  • Molecular weight

    240.35
  • Molecular formula

    C16H20N2
  • PubChem identifier

    41206
  • Storage instructions

    Store at +4°C short term (1-2 weeks). Store at -20°C. It is important to note that this product is reported to be light sensitive. Please see notes section. Stable for 12 months at -20°C.
  • Solubility overview

    Supplied in proprietary buffer with hydrogen peroxide (pH 3.6; 2.08 mM)
  • Handling

    Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Kabeya LM  et al. 3,3',5,5'-Tetramethylbenzidine in hypochlorous acid and taurine chloramine scavenging assays: interference of dimethyl sulfoxide and other vehicles. Anal Biochem 437:130-2 (2013). Read more (PubMed: 23499965) »
  • Josephy PD  et al. Cooxidation of the clinical reagent 3,5,3'5'-tetramethylbenzidine by prostaglandin synthase. Cancer Res 42:2567-70 (1982). Read more (PubMed: 6805942) »
  • Bos ES  et al. 3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay. J Immunoassay 2:187-204 (1981). Read more (PubMed: 7047570) »

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