Overview

  • Product name

    TMB, Peroxidase substrate (Ultrasensitive)
  • Description

    Peroxidase substrate (Ultrasensitive)
  • Alternative names

    • 3,3,5,5-Tetramethylbenzidine
  • General notes

    This material is extremely light sensitive. Take all appropriate steps to minimise exposure to all UV light sources, including sunlight. Discard if solution is blue or turbid.

  • CAS Number

    54827-17-7
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl
  • Molecular weight

    240.35
  • Molecular formula

    C16H20N2
  • PubChem identifier

    41206
  • Storage instructions

    Store at +4°C.
  • Solubility overview

    Supplied in citrate buffer with hydrogen peroxide (pH 3.3; 2.08 mM)
  • Handling

    Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Kabeya LM  et al. 3,3',5,5'-Tetramethylbenzidine in hypochlorous acid and taurine chloramine scavenging assays: interference of dimethyl sulfoxide and other vehicles. Anal Biochem 437:130-2 (2013). Read more (PubMed: 23499965) »
  • Josephy PD  et al. Cooxidation of the clinical reagent 3,5,3'5'-tetramethylbenzidine by prostaglandin synthase. Cancer Res 42:2567-70 (1982). Read more (PubMed: 6805942) »
  • Bos ES  et al. 3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay. J Immunoassay 2:187-204 (1981). Read more (PubMed: 7047570) »

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