Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19446] to TMC2
- Suitable for: WB, IHC-Fr, ICC/IF, Flow Cyt
- Reacts with: Mouse
Product nameAnti-TMC2 antibody [EPR19446]
See all TMC2 primary antibodies
DescriptionRabbit monoclonal [EPR19446] to TMC2
Tested applicationsSuitable for: WB, IHC-Fr, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse TMC2 aa 750 to the C-terminus. The exact sequence is proprietary.
Database link: Q8R4P4
- WB: HEK-293 transfected with DDDDK tagged mouse TMC2 expression vector, whole cell lysate. IHC-Fr: P21 Mouse cochlea tissue. ICC/IF: HEK-293T cells transfected with DDDDK tagged mouse TMC2 expression vector. Flow Cyt: HEK-293T transfected with DDDDK tagged mouse TMC2 expression vector.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab200039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).|
RelevanceThe specific function of this gene is unknown; however, expression in the inner ear suggests that it may be crucial for normal auditory function. Mutations in this gene may underlie hereditary disorders of balance and hearing.
Cellular localizationCell Membrane; Multi-pass membrane protein
- C20orf145 antibody
- dJ686C3.3 antibody
- Transmembrane channel like 2 antibody
All lanes : Anti-TMC2 antibody [EPR19446] (ab200039) at 1/5000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with a control vector containing DDDDK tag, whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with DDDDK tagged mouse TMC2 expression vector, whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 5 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
The Cell lysates were kindly provided by our collaborator Dr. Yun Shi. Nanjing University MARC.
The lysates were not boiled prior to loading on the gel.
Immunohistochemical analysis of 4% PFA-fixed whole mount P21 mouse cochlea tissue labeling TMC2 with ab200039 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (DyLight 488) secondary antibody at 1/500 dilution (green). The results showed cytoplasmic staining on mouse cochlea inner hair cells.
Red color: F-actin.
The data was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with DDDDK tagged mouse TMC2 expression vector, labeling TMC2 with ab200039 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HEK-293T cells transfected with DDDDK tagged mouse TMC2 expression vector.
The nuclear counter stain is DAPI (blue). FLAG is detected with Anti-FLAG® M2 (red) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 1/1000 dilution.
The negative controls are as follows:
-ve control 1: ab200039 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-FLAG® M2 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK tagged mouse TMC2 expression vector cell line labeling TMC2 with ab200039 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab200039 has not yet been referenced specifically in any publications.