Overview

  • Product name

    Anti-TMC2 antibody [EPR19446]
    See all TMC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19446] to TMC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse TMC2 aa 750 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8R4P4

  • Positive control

    • WB: HEK-293 transfected with DDDDK tagged mouse TMC2 expression vector, whole cell lysate. IHC-Fr: P21 Mouse cochlea tissue. ICC/IF: HEK-293T cells transfected with DDDDK tagged mouse TMC2 expression vector. Flow Cyt: HEK-293T transfected with DDDDK tagged mouse TMC2 expression vector.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19446
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
IHC-Fr 1/100.
ICC/IF 1/100.
Flow Cyt 1/50.

Target

  • Relevance

    The specific function of this gene is unknown; however, expression in the inner ear suggests that it may be crucial for normal auditory function. Mutations in this gene may underlie hereditary disorders of balance and hearing.
  • Cellular localization

    Cell Membrane; Multi-pass membrane protein
  • Database links

  • Alternative names

    • C20orf145 antibody
    • dJ686C3.3 antibody
    • Transmembrane channel like 2 antibody
    • Transmembrane channel like protein 2 antibody
    • Transmembrane cochlear expressed protein 2 antibody
    see all

Images

  • All lanes : Anti-TMC2 antibody [EPR19446] (ab200039) at 1/5000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with a control vector containing DDDDK tag, whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with DDDDK tagged mouse TMC2 expression vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 101 kDa
    Observed band size: 101 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The Cell lysates were kindly provided by our collaborator Dr. Yun Shi. Nanjing University MARC.

    The lysates were not boiled prior to loading on the gel.

  • Immunohistochemical analysis of 4% PFA-fixed whole mount P21 mouse cochlea tissue labeling TMC2 with ab200039  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (DyLight 488) secondary antibody at 1/500 dilution (green). The results showed cytoplasmic staining on mouse cochlea inner hair cells.

    Red color: F-actin.

    The data was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with DDDDK tagged mouse TMC2 expression vector, labeling TMC2 with ab200039 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HEK-293T cells transfected with DDDDK tagged mouse TMC2 expression vector.

    The nuclear counter stain is DAPI (blue). FLAG is detected with Anti-FLAG® M2 (red) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 1/1000 dilution.

    The negative controls are as follows:

    -ve control 1: ab200039 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 1/1000 dilution.

    -ve control 2: Anti-FLAG® M2 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK tagged mouse TMC2 expression vector cell line labeling TMC2 with ab200039 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

References

ab200039 has not yet been referenced specifically in any publications.

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