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    tmed4-antibody-ab153814.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway
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Anti-TMED4 antibody (ab153814)

  • Datasheet
  • SDS
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Western blot - Anti-TMED4 antibody (ab153814)
  • Immunocytochemistry/ Immunofluorescence - Anti-TMED4 antibody (ab153814)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMED4 antibody (ab153814)

Key features and details

  • Rabbit polyclonal to TMED4
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TMED4 antibody
  • Description

    Rabbit polyclonal to TMED4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-227 of Human TMED4.

  • Positive control

    • A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells, Human lung SCC tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis.
  • Sequence similarities

    Belongs to the EMP24/GP25L family.
    Contains 1 GOLD domain.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession Q7Z7H5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 508729 Cow
    • Entrez Gene: 222068 Human
    • Entrez Gene: 103694 Mouse
    • Entrez Gene: 444725 Xenopus laevis
    • Entrez Gene: 548496 Xenopus tropicalis
    • Entrez Gene: 415224 Zebrafish
    • Omim: 612038 Human
    • SwissProt: Q7Z7H5 Human
    • SwissProt: Q8R1V4 Mouse
    • Unigene: 598832 Human
    • Unigene: 715273 Human
    • Unigene: 254495 Mouse
    see all
  • Alternative names

    • Endoplasmic reticulum stress-response protein 25 antibody
    • ERS25 antibody
    • GMP25iso antibody
    • HNLF antibody
    • p24 family protein alpha-3 antibody
    • p24alpha3 antibody
    • Putative NF-kappa-B-activating protein 156 antibody
    • Putative NFkB activating protein HNLF antibody
    • TMED4 antibody
    • TMED4_HUMAN antibody
    • Transmembrane emp24 domain containing protein 4 antibody
    • Transmembrane emp24 domain-containing protein 4 antibody
    • Transmembrane emp24 protein transport domain containing 4 antibody
    see all

Images

  • Western blot - Anti-TMED4 antibody (ab153814)
    Western blot - Anti-TMED4 antibody (ab153814)
    Anti-TMED4 antibody (ab153814) at 1/1000 dilution + HepG2 whole cell
    lysate at 30 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-TMED4 antibody (ab153814)
    Immunocytochemistry/ Immunofluorescence - Anti-TMED4 antibody (ab153814)
    Immunofluorescent analysis of methanol-fixed HeLa cells labeling TMED4 with ab153814 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMED4 antibody (ab153814)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMED4 antibody (ab153814)
    Immunohistochemical analysis of paraffin-embedded Human lung SCC tissue labeling TMED4 with ab153814 at 1/100 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab153814? Please let us know so that we can cite the reference in this datasheet.

ab153814 has not yet been referenced specifically in any publications.

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