Anti-TMED4 antibody (ab153814)
Key features and details
- Rabbit polyclonal to TMED4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TMED4 antibody -
Description
Rabbit polyclonal to TMED4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 1-227 of Human TMED4.
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Positive control
- A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells, Human lung SCC tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis. -
Sequence similarities
Belongs to the EMP24/GP25L family.
Contains 1 GOLD domain. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 508729 Cow
- Entrez Gene: 222068 Human
- Entrez Gene: 103694 Mouse
- Entrez Gene: 444725 Xenopus laevis
- Entrez Gene: 548496 Xenopus tropicalis
- Entrez Gene: 415224 Zebrafish
- Omim: 612038 Human
- SwissProt: Q7Z7H5 Human
see all -
Alternative names
- Endoplasmic reticulum stress-response protein 25 antibody
- ERS25 antibody
- GMP25iso antibody
see all
Images
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Western blot - Anti-TMED4 antibody (ab153814)Anti-TMED4 antibody (ab153814) at 1/1000 dilution + HepG2 whole cell
lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-TMED4 antibody (ab153814)Immunofluorescent analysis of methanol-fixed HeLa cells labeling TMED4 with ab153814 at 1/200 dilution. Lower panel co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMED4 antibody (ab153814)Immunohistochemical analysis of paraffin-embedded Human lung SCC tissue labeling TMED4 with ab153814 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab153814 has not yet been referenced specifically in any publications.