Anti-TMED7 antibody (ab126094)

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Western blot - Anti-TMED7 antibody (ab126094)

    Key features and details

    • Rabbit polyclonal to TMED7
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-TMED7 antibody

    • Description

      Rabbit polyclonal to TMED7

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Recombinant fragment, corresponding to a region within amino acids 12-196 of the Human TMED7 protein (Q9Y3B3).

    • Positive control

      • 293T and A431 cell lysate.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab126094 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Rat
    Cow
    Xenopus laevis
    Zebrafish
    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 25 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 25 kDa.

    Target

    Images

    • Western blot - Anti-TMED7 antibody (ab126094)
      Western blot - Anti-TMED7 antibody (ab126094)
      All lanes : Anti-TMED7 antibody (ab126094) at 1/1000 dilution

      Lane 1 : 293T whole cell lysate
      Lane 2 : A431 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 25 kDa



      Gel concentration: 12%

    References (0)

    Publishing research using ab126094? Please let us know so that we can cite the reference in this datasheet.

    ab126094 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Submit a review Submit a question

    Question

    I'm using this antibody to detect TMED7 in HEK293T cell lysate but only seeing a very weak band at 25 kDa. Do you have a recommended protocol?

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    Answer

    Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

    I am attaching the requested protocol. If you try this protocol and it doesn't work, please let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

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