Product nameAnti-TMEFF2 antibody [EPR6385]
See all TMEFF2 primary antibodies
DescriptionRabbit monoclonal [EPR6385] to TMEFF2
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in the extracellular domain in TMEFF2 (Human)
- HeLa, SW480 or Hman prostate lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab124775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 41 kDa).|
FunctionMay be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
Tissue specificityHighly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques (at protein level). Isoform 3 is expressed weakly in testis and at high levels in normal and cancerous prostate.
Sequence similaritiesBelongs to the tomoregulin family.
Contains 1 EGF-like domain.
Contains 2 Kazal-like domains.
modificationsN-glycosylated. Contains chondroitin sulfate glycosaminoglycans.
A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or proinflammatory cytokines such as TNFalpha, and is mediated by ADAM17.
Cellular localizationSecreted and Membrane.
- Information by UniProt
- Cancer/testis antigen family 120 member 2 antibody
- CT120.2 antibody
- HPP 1 antibody
All lanes : Anti-TMEFF2 antibody [EPR6385] (ab124775) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : SW480 lysate
Lane 3 : Human prostate lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
ab124775 has not yet been referenced specifically in any publications.