Recombinant
RabMAb

Recombinant Anti-TMEFF2 antibody [EPR6385] (ab124775)

Overview

  • Product name

    Anti-TMEFF2 antibody [EPR6385]
    See all TMEFF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6385] to TMEFF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the extracellular domain in TMEFF2 (Human)

  • Positive control

    • HeLa, SW480 or Hman prostate lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6385
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 41 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
    • Tissue specificity

      Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques (at protein level). Isoform 3 is expressed weakly in testis and at high levels in normal and cancerous prostate.
    • Sequence similarities

      Belongs to the tomoregulin family.
      Contains 1 EGF-like domain.
      Contains 2 Kazal-like domains.
    • Post-translational
      modifications

      N-glycosylated. Contains chondroitin sulfate glycosaminoglycans.
      A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or proinflammatory cytokines such as TNFalpha, and is mediated by ADAM17.
    • Cellular localization

      Secreted and Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cancer/testis antigen family 120 member 2 antibody
      • CT120.2 antibody
      • HPP 1 antibody
      • HPP1 antibody
      • Hyperplastic polyposis protein 1 antibody
      • TEFF 2 antibody
      • TEFF2 antibody
      • TEFF2_HUMAN antibody
      • TENB 2 antibody
      • TENB2 antibody
      • TMEFF2 antibody
      • Tomoregulin antibody
      • Tomoregulin-2 antibody
      • TPEF antibody
      • TR 2 antibody
      • TR antibody
      • TR-2 antibody
      • Transmembrane protein containing epidermal growth factor and follistatin domains antibody
      • Transmembrane protein TENB2 antibody
      • Transmembrane protein with EGF like and two follistatin like domains 2 antibody
      • Transmembrane protein with EGF-like and two follistatin-like domains antibody
      see all

    Images

    • All lanes : Anti-TMEFF2 antibody [EPR6385] (ab124775) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : SW480 lysate
      Lane 3 : Human prostate lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution

      Predicted band size: 41 kDa
      Observed band size: 44 kDa
      why is the actual band size different from the predicted?

    References

    ab124775 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Question
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the newest lot of this product with the order number xxxx. Include in this order a free RabMAb anti-TMEFF2 as well. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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