Recombinant
RabMAb

Recombinant Anti-TMEFF2 antibody [EPR6386] (ab133562)

Overview

  • Product name

    Anti-TMEFF2 antibody [EPR6386]
    See all TMEFF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6386] to TMEFF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region in the intracellular domain within the C-terminus of Human TMEFF2 (Q9UIK5).

  • Positive control

    • Human prostatic hyperplasia tissue; HeLa, LNCaP, Caco 2 and SW480 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.68 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6386
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
    • Tissue specificity

      Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques (at protein level). Isoform 3 is expressed weakly in testis and at high levels in normal and cancerous prostate.
    • Sequence similarities

      Belongs to the tomoregulin family.
      Contains 1 EGF-like domain.
      Contains 2 Kazal-like domains.
    • Post-translational
      modifications

      N-glycosylated. Contains chondroitin sulfate glycosaminoglycans.
      A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or proinflammatory cytokines such as TNFalpha, and is mediated by ADAM17.
    • Cellular localization

      Secreted and Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cancer/testis antigen family 120 member 2 antibody
      • CT120.2 antibody
      • HPP 1 antibody
      • HPP1 antibody
      • Hyperplastic polyposis protein 1 antibody
      • TEFF 2 antibody
      • TEFF2 antibody
      • TEFF2_HUMAN antibody
      • TENB 2 antibody
      • TENB2 antibody
      • TMEFF2 antibody
      • Tomoregulin antibody
      • Tomoregulin-2 antibody
      • TPEF antibody
      • TR 2 antibody
      • TR antibody
      • TR-2 antibody
      • Transmembrane protein containing epidermal growth factor and follistatin domains antibody
      • Transmembrane protein TENB2 antibody
      • Transmembrane protein with EGF like and two follistatin like domains 2 antibody
      • Transmembrane protein with EGF-like and two follistatin-like domains antibody
      see all

    Images

    • All lanes : Anti-TMEFF2 antibody [EPR6386] (ab133562) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : LnCaP cell lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 41 kDa

    • Immunohistochemical analysis of formailin-fixed, paraffin-embedded Human prostatic hyperplasia tissue staining TMEFF2 using ab133562 at a 1/50 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133562 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133562.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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