Product nameAnti-TMEFF2 antibody [EPR6386]
See all TMEFF2 primary antibodies
DescriptionRabbit monoclonal [EPR6386] to TMEFF2
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region in the intracellular domain within the C-terminus of Human TMEFF2 (Q9UIK5).
- Human prostatic hyperplasia tissue; HeLa, LNCaP, Caco 2 and SW480 cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.68 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Predicted molecular weight: 41 kDa.|
FunctionMay be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
Tissue specificityHighly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques (at protein level). Isoform 3 is expressed weakly in testis and at high levels in normal and cancerous prostate.
Sequence similaritiesBelongs to the tomoregulin family.
Contains 1 EGF-like domain.
Contains 2 Kazal-like domains.
modificationsN-glycosylated. Contains chondroitin sulfate glycosaminoglycans.
A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or proinflammatory cytokines such as TNFalpha, and is mediated by ADAM17.
Cellular localizationSecreted and Membrane.
- Information by UniProt
- Cancer/testis antigen family 120 member 2 antibody
- CT120.2 antibody
- HPP 1 antibody
All lanes : Anti-TMEFF2 antibody [EPR6386] (ab133562) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Immunohistochemical analysis of formailin-fixed, paraffin-embedded Human prostatic hyperplasia tissue staining TMEFF2 using ab133562 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133562 has not yet been referenced specifically in any publications.