Recombinant
RabMAb

Recombinant Anti-TMEFF2 antibody [EPR6386] - BSA and Azide free (ab248554)

Overview

  • Product name

    Anti-TMEFF2 antibody [EPR6386] - BSA and Azide free
    See all TMEFF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6386] to TMEFF2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TMEFF2 aa 350-450 (C terminal). The exact sequence is proprietary. A region in the intracellular domain within the C-terminus.
    Database link: Q9UIK5

  • General notes

    ab248554 is the carrier-free version of ab133562 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248554 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.68 x 10 -10 M
    Learn more about KD
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6386
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab248554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
    • Tissue specificity

      Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques (at protein level). Isoform 3 is expressed weakly in testis and at high levels in normal and cancerous prostate.
    • Sequence similarities

      Belongs to the tomoregulin family.
      Contains 1 EGF-like domain.
      Contains 2 Kazal-like domains.
    • Post-translational
      modifications

      N-glycosylated. Contains chondroitin sulfate glycosaminoglycans.
      A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or proinflammatory cytokines such as TNFalpha, and is mediated by ADAM17.
    • Cellular localization

      Secreted and Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cancer/testis antigen family 120 member 2 antibody
      • CT120.2 antibody
      • HPP 1 antibody
      • HPP1 antibody
      • Hyperplastic polyposis protein 1 antibody
      • TEFF 2 antibody
      • TEFF2 antibody
      • TEFF2_HUMAN antibody
      • TENB 2 antibody
      • TENB2 antibody
      • TMEFF2 antibody
      • Tomoregulin antibody
      • Tomoregulin-2 antibody
      • TPEF antibody
      • TR 2 antibody
      • TR antibody
      • TR-2 antibody
      • Transmembrane protein containing epidermal growth factor and follistatin domains antibody
      • Transmembrane protein TENB2 antibody
      • Transmembrane protein with EGF like and two follistatin like domains 2 antibody
      • Transmembrane protein with EGF-like and two follistatin-like domains antibody
      see all

    References

    ab248554 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab248554.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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