Product nameAnti-TMEFF2 antibody [EPR6386] - BSA and Azide free
See all TMEFF2 primary antibodies
DescriptionRabbit monoclonal [EPR6386] to TMEFF2 - BSA and Azide free
Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human TMEFF2 aa 350-450 (C terminal). The exact sequence is proprietary. A region in the intracellular domain within the C-terminus.
Database link: Q9UIK5
ab248554 is the carrier-free version of ab133562 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248554 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 1.68 x 10 -10 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.|
FunctionMay be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
Tissue specificityHighly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques (at protein level). Isoform 3 is expressed weakly in testis and at high levels in normal and cancerous prostate.
Sequence similaritiesBelongs to the tomoregulin family.
Contains 1 EGF-like domain.
Contains 2 Kazal-like domains.
modificationsN-glycosylated. Contains chondroitin sulfate glycosaminoglycans.
A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or proinflammatory cytokines such as TNFalpha, and is mediated by ADAM17.
Cellular localizationSecreted and Membrane.
- Information by UniProt
- Cancer/testis antigen family 120 member 2 antibody
- CT120.2 antibody
- HPP 1 antibody
ab248554 has not yet been referenced specifically in any publications.