Overview

  • Product name

  • Description

    Rabbit polyclonal to TMEM106B
  • Host species

    Rabbit
  • Specificity

    ab140185 is predicted to not cross-react with TMEM106A.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A 15 amino acid synthetic peptide near the N terminus of Human TMEM106B.

  • Positive control

    • Human Liver tissue and lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab140185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Tissue specificity

    Expressed in frontal cortex.
  • Involvement in disease

    Note=TMEM106B genotype, when containing 3 particular single-nucleotide polymorphisms, is strongly correlated with frontotemporal lobar degeneration with TAR DNA-binding protein (TDP-43) inclusions (FTLD-TDP). Frontotemporal lobar degeneration (FTLD) is the second most common cause of presenile dementia and 20% of patients with this neurodegenerative disease have autosomal dominant GRN mutations. Expression of TMEM106B associated with these polymorphisms is increased in frontal cortex of patients with FTLD-TDP compared to unaffected controls. Thus, increased TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.
  • Sequence similarities

    Belongs to the TMEM106 family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2310036D22Rik antibody
    • 5830455K21Rik antibody
    • 6430519M21Rik antibody
    • AI428776 antibody
    • AI661344 antibody
    • FLJ11273 antibody
    • LRRGT00101 antibody
    • MGC33727 antibody
    • MGC94135 antibody
    • T106B_HUMAN antibody
    • Tmem106b antibody
    • Transmembrane protein 106B antibody
    see all

Images

  • All lanes : Anti-TMEM106B antibody (ab140185) at 1 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate with Immunizing peptide

    Predicted band size: 31 kDa

  • Immunohistochemical staining of TMEM106B in Human liver tissue with ab140185 at 20 ug/mL.

  • Anti-TMEM106B antibody (ab140185) at 1 µg/ml + Human Liver tissue lysate at 15 µg

    Predicted band size: 31 kDa

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling TMEM106B with ab140185 at 2.5 µg/ml.

References

ab140185 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10-20%)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 06 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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