Overview

  • Product name

    Anti-TMEM107 antibody - N-terminal
  • Description

    Rabbit polyclonal to TMEM107 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TMEM107 (N terminal). (19 amino acid peptide; NP_115730).
    Database link: Q6UX40-4

  • Positive control

    • THP-1 cell lysate; Human lymph node tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 16 kDa).

Target

Images

  • All lanes : Anti-TMEM107 antibody - N-terminal (ab181396) at 1 µg/ml

    Lane 1 : THP-1 cell lysate
    Lane 2 : THP-1 cell lysate with immunizing peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 16 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling TMEM107 with ab181396 at 5 µg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling TMEM107 with ab181396 at 20 µg/ml.

References

This product has been referenced in:

  • Jenkinson EM  et al. Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts. Nat Genet 48:1185-92 (2016). Read more (PubMed: 27571260) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hTERT RPE-1)
Permeabilization
No
Specification
hTERT RPE-1
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Methanol

Alice Lake

Verified customer

Submitted Oct 10 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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