Overview

  • Product name

    Anti-TMEM109/Mitsugumin-23 antibody
    See all TMEM109/Mitsugumin-23 primary antibodies
  • Description

    Rabbit polyclonal to TMEM109/Mitsugumin-23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human TMEM109/Mitsugumin-23 aa 212-242.
    Sequence:

    LEAKVRGLERQVEELRWRQRRAAKGARSVEE

  • Positive control

    • Human Pancreas tissue; U 251 MG, RT4 lysate, Human Liver and Tonsil lysates; U 251 MG cells.
  • General notes

     This product was previously labelled as TMEM109

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. using Fixation/Permeabilization: PFA/Triton X-100.

Target

Images

  • All lanes : Anti-TMEM109/Mitsugumin-23 antibody (ab121349) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique.
  • ab121349, at 1/1000, staining TMEM109/Mitsugumin-23 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
    Note: strong cytoplasmic positivity in exocrine glandular cells.

  • ab121349 at 4 µg/ml staining TMEM109/Mitsugumin-23 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
    Note: positivity in nucleus but not nucleoli, nuclear membrane and cytoplasm.

References

ab121349 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Human Cell (Prostate)
Specification
Prostate
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 13 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Prostate)
Permeabilization
No
Specification
Prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Aug 13 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate)
Loading amount
25 µg
Specification
Prostate
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 10 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Prostate cell line LNCaP)
Permeabilization
No
Specification
Prostate cell line LNCaP
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 10 2012

Question
Answer

Thank you for contacting us.

The antibody has been tested in WB, ICC/IF and IHC-P with human samples. This antibody is fully guaranteed to be used in these listed applications and species.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up