Anti-TMEM109/Mitsugumin-23 antibody (ab241992)
Key features and details
- Rabbit polyclonal to TMEM109/Mitsugumin-23
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM109/Mitsugumin-23 antibody
See all TMEM109/Mitsugumin-23 primary antibodies -
Description
Rabbit polyclonal to TMEM109/Mitsugumin-23 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human TMEM109/Mitsugumin-23 aa 25-75. The exact sequence is proprietary.
Database link: Q9BVC6 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: TMEM109/Mitsugumin-23 IP in HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to TMEM109/Mitsugumin-23 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000.
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IP |
Use at an assay dependent concentration.
20 μl/mg lysate
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Notes |
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WB
1/1000. |
IP
Use at an assay dependent concentration. 20 μl/mg lysate
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Target
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Cellular localization
Nucleus outer membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 79073 Human
- Entrez Gene: 68539 Mouse
- SwissProt: Q9BVC6 Human
- SwissProt: Q3UBX0 Mouse
- Unigene: 13662 Human
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Alternative names
- Mg23 antibody
- Mitsugumin-23 antibody
- TM109_HUMAN antibody
see all
Images
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All lanes : Anti-TMEM109/Mitsugumin-23 antibody (ab241992) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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TMEM109/Mitsugumin-23 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241992 at 20 µL per reaction. Western blot was performed from the immunoprecipitate using ab241992 at 1/100 dilution.
Lane 1: ab241992 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
Prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab241992 has not yet been referenced specifically in any publications.