Key features and details
- Rabbit polyclonal to TMEM109/Mitsugumin-23
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TMEM109/Mitsugumin-23 antibody
See all TMEM109/Mitsugumin-23 primary antibodies
DescriptionRabbit polyclonal to TMEM109/Mitsugumin-23
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human TMEM109/Mitsugumin-23 aa 25-75. The exact sequence is proprietary.
Database link: Q9BVC6
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: TMEM109/Mitsugumin-23 IP in HEK-293T whole cell lysate.
This product was previously labelled as TMEM109
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to TMEM109/Mitsugumin-23 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 μl/mg lysate
Cellular localizationNucleus outer membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
- Information by UniProt
- Mg23 antibody
- Mitsugumin-23 antibody
- TM109_HUMAN antibody
All lanes : Anti-TMEM109/Mitsugumin-23 antibody (ab241992) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
TMEM109/Mitsugumin-23 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241992 at 20 µL per reaction. Western blot was performed from the immunoprecipitate using ab241992 at 1/100 dilution.
Lane 1: ab241992 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241992 has not yet been referenced specifically in any publications.