Product nameAnti-TMEM109/Mitsugumin-23 antibody
See all TMEM109/Mitsugumin-23 primary antibodies
DescriptionRabbit polyclonal to TMEM109/Mitsugumin-23
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TMEM109/Mitsugumin-23 aa 25-75. The exact sequence is proprietary.
Database link: Q9BVC6
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
This product was previously labelled as TMEM109
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to TMEM109/Mitsugumin-23 immobilized on solid support.
Our Abpromise guarantee covers the use of ab242096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 26 kDa.|
|IP||Use at an assay dependent concentration.
Cellular localizationNucleus outer membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
- Information by UniProt
- Mg23 antibody
- Mitsugumin-23 antibody
- TM109_HUMAN antibody
TMEM109/Mitsugumin-23 IP in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242096 at 20 µL per reaction. Western blot was performed from the immunoprecipitate using ab242096 at 1/250 dilution.
Lane 1: ab242096 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Lysate prepared in NETN buffer.
All lanes : Anti-TMEM109/Mitsugumin-23 antibody (ab242096) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 26 kDa
Exposure time: 30 seconds
Lysates prepared in NETN buffer.
ab242096 has not yet been referenced specifically in any publications.