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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

Submit a review Submit a question References (1)
Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
  • Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [106-6] to TMEM119 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 PE Unconjugated

Overview

  • Product name

    Anti-TMEM119 antibody [106-6] - BSA and Azide free
    See all TMEM119 primary antibodies

  • Description

    Rabbit monoclonal [106-6] to TMEM119 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells).

  • General notes

    ab220249 is the carrier-free version of ab210405.

    Please note that the original Bennett et al . (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author's permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab220249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use a concentration of 0.1 - 0.5 µg/ml.

For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474
Notes
Flow Cyt
Use a concentration of 0.1 - 0.5 µg/ml.

For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474

Target

Images

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells  labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.

    No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).

    The data was provided by Ben Barres’ lab (Stanford University).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (Alexa Fluor® 488). Please refer to ab225497 for protocol details.

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225497 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 488 ab199091 (left). Cells were surface-stained with ab225497, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (PE). Please refer to ab225496 for protocol details.

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225496 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Phycoerythrin ab209478 (left). Cells were surface-stained with ab225496, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (Alexa Fluor® 647). Please refer to ab225494 for protocol details.

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225494 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 647 ab199093 (left). Cells were surface-stained with ab225494, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 488 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

  • Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
    Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab220249? Please let us know so that we can cite the reference in this datasheet.

ab220249 has been referenced in 1 publication.

  • Bennett ML  et al. New tools for studying microglia in the mouse and human CNS. Proc Natl Acad Sci U S A 113:E1738-46 (2016). PubMed: 26884166

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