Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [106-6] to TMEM119 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Mouse

Overview

  • Product name

    Anti-TMEM119 antibody [106-6] - BSA and Azide free
    See all TMEM119 primary antibodies

  • Description

    Rabbit monoclonal [106-6] to TMEM119 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: Q8R138

  • Positive control

    • Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells).

  • General notes

    ab220249 is the carrier-free version of ab210405 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab220249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Please note that the original Bennett et al . (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author&rsquo;s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab220249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.1 - 0.5 µg/ml.

For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474

Target

  • Cellular localization

    Membrane; Single-pass type I membrane protein

  • Database links

    • Alternative names

      • OBIF antibody
      • Osteoblast induction factor antibody
      • PSEC0199 antibody
      • Transmembrane protein 119 antibody
      • UNQ731/PRO1415 antibody
      see all

    Images

    • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
      Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

      Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).

    • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
      Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

      Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells  labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.

      No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).

      The data was provided by Ben Barres’ lab (Stanford University).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).

    • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
      Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

      Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (Alexa Fluor® 488). Please refer to ab225497 for protocol details.

      Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225497 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 488 ab199091 (left). Cells were surface-stained with ab225497, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

    • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
      Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

      Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (PE). Please refer to ab225496 for protocol details.

      Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225496 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Phycoerythrin ab209478 (left). Cells were surface-stained with ab225496, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

    • Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
      Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

      Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (Alexa Fluor® 647). Please refer to ab225494 for protocol details.

      Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225494 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 647 ab199093 (left). Cells were surface-stained with ab225494, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 488 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

    • Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
      Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab220249? Please let us know so that we can cite the reference in this datasheet.

    ab220249 has been referenced in 1 publication.

    • Bennett ML  et al. New tools for studying microglia in the mouse and human CNS. Proc Natl Acad Sci U S A 113:E1738-46 (2016). PubMed: 26884166

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