Recombinant Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)
Knockout Tested Rabbit recombinant monoclonal TMEM119 antibody [106-6]. Validated in Flow Cyt and tested in Mouse. Cited in 1 publication(s).
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-TMEM119 antibody [106-6] - BSA and Azide free
See all TMEM119 primary antibodies -
Description
Rabbit monoclonal [106-6] to TMEM119 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact sequence is proprietary.
Database link: Q8R138 -
Positive control
- Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells).
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General notes
ab220249 is the carrier-free version of ab210405 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab220249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Please note that the original Bennett et al . (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author’s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
106-6 -
Isotype
IgG
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab220249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use a concentration of 0.1 - 0.5 µg/ml. For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474 |
Target
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Cellular localization
Membrane; Single-pass type I membrane protein -
Database links
- Entrez Gene: 231633 Mouse
- SwissProt: Q8R138 Mouse
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Alternative names
- OBIF antibody
- Osteoblast induction factor antibody
- PSEC0199 antibody
see all
Images
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![Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)](https://www.abcam.com/ps/products/220/ab220249/Images/ab220249-321129-anti-tmem119-antibody-106-6-flow-cytometry.jpg)
Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).
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![Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)](https://www.abcam.com/ps/products/220/ab220249/Images/ab220249-321128-anti-tmem119-antibody-106-6-flow-cytometry.jpg)
Flow Cytometry - Anti-TMEM119 antibody [106-6] - BSA and Azide free (ab220249)Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.
No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).
The data was provided by Ben Barres’ lab (Stanford University).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).
Datasheets and documents
References
This product has been referenced in:
- Bennett ML et al. New tools for studying microglia in the mouse and human CNS. Proc Natl Acad Sci U S A 113:E1738-46 (2016). Read more (PubMed: 26884166) »
