Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [106-6] to TMEM119 - Microglial marker
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Mouse
Product nameAnti-TMEM119 antibody [106-6] - Microglial marker
See all TMEM119 primary antibodies
DescriptionRabbit monoclonal [106-6] to TMEM119 - Microglial marker
Human reactivity has not been tested.
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: IHC-Fr or IHC-P
Species reactivityReacts with: Mouse
Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact sequence is proprietary.
Database link: Q8R138
- Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells), HEK-293T transfected with Myc-His tagged TMEM119
Please note, this antibody is suitable for flow cytometry. For IHC on mouse brain tissue we recommend ab209064.
Please note that the original Bennett et al. (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author’s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.
The 106-6 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab210405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 0.1 - 0.5 µg/ml.
For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474
Cellular localizationMembrane; Single-pass type I membrane protein
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Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.
Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.
No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).
The data was provided by Ben Barres’ lab (Stanford University).
ab210405 has been referenced in 8 publications.
- Ham SW et al. TP53 gain-of-function mutation promotes inflammation in glioblastoma. Cell Death Differ 26:409-425 (2019). PubMed: 29786075
- Choi JY et al. Indispensable Role of CX3CR1+ Dendritic Cells in Regulation of Virus-Induced Neuroinflammation Through Rapid Development of Antiviral Immunity in Peripheral Lymphoid Tissues. Front Immunol 10:1467 (2019). PubMed: 31316515
- Dusoswa SA et al. OMIP-054: Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass Cytometry. Cytometry A 95:422-426 (2019). PubMed: 30701669
- Li Q et al. Developmental Heterogeneity of Microglia and Brain Myeloid Cells Revealed by Deep Single-Cell RNA Sequencing. Neuron 101:207-223.e10 (2019). PubMed: 30606613
- Chhatbar C et al. Type I Interferon Receptor Signaling of Neurons and Astrocytes Regulates Microglia Activation during Viral Encephalitis. Cell Rep 25:118-129.e4 (2018). PubMed: 30282022
- Vandermosten L et al. Adrenal hormones mediate disease tolerance in malaria. Nat Commun 9:4525 (2018). PubMed: 30375380
- Deliyanti D et al. Foxp3+ Tregs are recruited to the retina to repair pathological angiogenesis. Nat Commun 8:748 (2017). Flow Cyt ; Mouse . PubMed: 28963474
- Bennett ML et al. New tools for studying microglia in the mouse and human CNS. Proc Natl Acad Sci U S A 113:E1738-46 (2016). PubMed: 26884166