Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)

Overview

  • Product name

    Anti-TMEM119 antibody [106-6] - Microglial marker
    See all TMEM119 primary antibodies
  • Description

    Rabbit monoclonal [106-6] to TMEM119 - Microglial marker
  • Host species

    Rabbit
  • Specificity

    Human reactivity has not been tested.

  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: IHC-Fr or IHC-P
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: Q8R138

  • Positive control

    • Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells), HEK-293T transfected with Myc-His tagged TMEM119
  • General notes

    Please note, this antibody is suitable for flow cytometry. For IHC on mouse brain tissue we recommend ab209064.

    Please note that the original Bennett et al. (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author’s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.

    The 106-6 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    106-6
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab210405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.1 - 0.5 µg/ml.

For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474

  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • Target

    • Cellular localization

      Membrane; Single-pass type I membrane protein
    • Database links

    • Alternative names

      • OBIF antibody
      • Osteoblast induction factor antibody
      • PSEC0199 antibody
      • Transmembrane protein 119 antibody
      • UNQ731/PRO1415 antibody
      see all

    Images

    • Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells  labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.

      No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).

      The data was provided by Ben Barres’ lab (Stanford University).

    • Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.

    References

    This product has been referenced in:

    • Ham SW  et al. TP53 gain-of-function mutation promotes inflammation in glioblastoma. Cell Death Differ 26:409-425 (2019). Read more (PubMed: 29786075) »
    • Deliyanti D  et al. Foxp3+ Tregs are recruited to the retina to repair pathological angiogenesis. Nat Commun 8:748 (2017). Flow Cyt ; Mouse . Read more (PubMed: 28963474) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Application
    Flow Cytometry
    Sample
    Mouse Cell (Primary Microglia)
    Permeabilization
    No
    Gating Strategy
    Gated on live CD11b+/CD45low cells
    Specification
    Primary Microglia
    Preparation
    Cell harvesting/tissue preparation method: Processed using Miltenyi Neural tissue digest kit (with and without enzyme P then myelin depleted using Miltenyi magnetic beads
    Sample buffer: NTDK +/- enzyme P (suspected papain) in HBSS
    Fixation
    Live cells

    Abcam user community

    Verified customer

    Submitted Mar 29 2018

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate 0,01M
    Permeabilization
    Yes - PBS 0,1M triton 0,1%
    Specification
    spinal cord
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 05 2017

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Temporal Cortex)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Temporal Cortex
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formalin

    Mrs. Julie Castro

    Verified customer

    Submitted Jul 10 2017

    Application
    Flow Cytometry
    Sample
    Rat Cell (Cortex)
    Permeabilization
    No
    Gating Strategy
    CD11b+ Microglia
    Specification
    Cortex
    Preparation
    Cell harvesting/tissue preparation method: Microglia were isolated by CD11b magnetic bead isolation from the cortex of adult, female SD rats.
    Sample buffer: PBS/0.5% BSA
    Fixation
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 25 2017

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue (Brain, microglia)
    Permeabilization
    Yes - 0.3% Triton-X in PBS
    Specification
    Brain, microglia
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 05 2017

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Brain
    Blocking step
    CytoQ Immunodiluent & Block as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Fixative
    Paraformaldehyde

    Sk De

    Verified customer

    Submitted Mar 14 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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