Recombinant Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
![Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)](https://www.abcam.com/ps/products/210/ab210405/Images/ab210405-297063-anti-tmem119-antibody-106-6-microglial-marker-flow-cytometry.jpg "Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [106-6] to TMEM119 - Microglial marker
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TMEM119 antibody [106-6] - Microglial marker
See all TMEM119 primary antibodies -
Description
Rabbit monoclonal [106-6] to TMEM119 - Microglial marker -
Host species
Rabbit -
Specificity
Human reactivity has not been tested.
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Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells), HEK-293T transfected with Myc-His tagged TMEM119
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General notes
Please note, this antibody is suitable for flow cytometry. For IHC on mouse brain tissue we recommend ab209064.
Please note that the original Bennett et al. (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author’s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.
The 106-6 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
106-6 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (2) |
Use a concentration of 0.1 - 0.5 µg/ml.
For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474 |
| Notes |
|---|
|
Flow Cyt
Use a concentration of 0.1 - 0.5 µg/ml. For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474 |
Target
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Cellular localization
Membrane; Single-pass type I membrane protein -
Database links
- Entrez Gene: 338773 Human
- Entrez Gene: 231633 Mouse
- SwissProt: Q4V9L6 Human
- SwissProt: Q8R138 Mouse
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Alternative names
- OBIF antibody
- Osteoblast induction factor antibody
- PSEC0199 antibody
see all
Images
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Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.
Alexa Fluor® 488 (ab225497) and R-PE (ab225496) conjugated versions are available for this clone.
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![Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)](https://www.abcam.com/ps/products/210/ab210405/Images/ab210405-272564-anti-tmem119-antibody-106-6-microglial-marker-flow-cytometry.jpg)
Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.
No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).
The data was provided by Ben Barres’ lab (Stanford University).
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![Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)](https://www.abcam.com/ps/products/210/ab210405/Images/ab210405-5-benefits-of-recombinant-antibodies.png)
Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (12)
ab210405 has been referenced in 12 publications.
- Rong K et al. Alendronate Alleviated Femoral Head Necrosis and Upregulated BMP2/EIF2AK3/EIF2A/ATF4 Pathway in Liquid Nitrogen Treated Rats. Drug Des Devel Ther 15:1717-1724 (2021). PubMed: 33935494
- Kodama L et al. Microglial microRNAs mediate sex-specific responses to tau pathology. Nat Neurosci 23:167-171 (2020). PubMed: 31873194
- Bohlen CJ et al. Isolation and Culture of Microglia. Curr Protoc Immunol 125:e70 (2019). PubMed: 30414379
- Figueiredo CP et al. Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice. Nat Commun 10:3890 (2019). PubMed: 31488835
- Ham SW et al. TP53 gain-of-function mutation promotes inflammation in glioblastoma. Cell Death Differ 26:409-425 (2019). PubMed: 29786075