Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Brain
Blocking step
CytoQ Immunodiluent & Block as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 647-AffiniPure Goat Anti-Rabbit IgG (H
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Additional data
Additional Notes
This Tmem119 primary antibody seems to label (magenta, Fig. 1) all microglia (green, Fig. 1) in mouse brain sections, when used for immunohistochemistry (frozen). Macrophages in the brain vasculature and meninges (marked by white dashed line, Fig. 1) were mostly not labeled. However, certain sections showed high background (Fig. 2) and non-specific labeling (white asterisk, Fig. 2).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sk De
Verified customer
Submitted Mar 14 2017