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    tmem119-antibody-c-terminal-ab185333.pdf

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Anti-TMEM119 antibody - C-terminal (ab185333)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (18)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)

Key features and details

  • Rabbit polyclonal to TMEM119 - C-terminal
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TMEM119 antibody - C-terminal
    See all TMEM119 primary antibodies
  • Description

    Rabbit polyclonal to TMEM119 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TMEM119 aa 150 to the C-terminus (C terminal).
    Database link: Q4V9L6
    (Peptide available as ab207307)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    This antibody is suitable for detecting human TMEM119 by IHC. To detect mouse TMEM119 by IHC, we recommend using ab209064.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • TMEM119 peptide (ab207307)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Cellular localization

    Membrane; Single-pass type I membrane protein
  • Database links

    • Entrez Gene: 338773 Human
    • SwissProt: Q4V9L6 Human
    • Alternative names

      • OBIF antibody
      • Osteoblast induction factor antibody
      • PSEC0199 antibody
      • Transmembrane protein 119 antibody
      • UNQ731/PRO1415 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling TMEM119 with ab185333 at a dilution of 1/1000.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling TMEM119 with ab185333 at a dilution of 1/1000.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling TMEM119 with ab185333 at a dilution of 1/1000.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal (ab185333)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling TMEM119 with ab185333 at a dilution of 1/1000.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (18)

    Publishing research using ab185333? Please let us know so that we can cite the reference in this datasheet.

    ab185333 has been referenced in 18 publications.

    • Awad H  et al. Endovascular repair and open repair surgery of thoraco-abdominal aortic aneurysms cause drastically different types of spinal cord injury. Sci Rep 11:7834 (2021). PubMed: 33837260
    • Schwabenland M  et al. Analyzing microglial phenotypes across neuropathologies: a practical guide. Acta Neuropathol N/A:N/A (2021). PubMed: 34623511
    • Zhang J  et al. Human Microglia Extensively Reconstitute in Humanized-BLT Mice With Human Interleukin-34 Transgene and Support HIV-1 Brain Infection. Front Immunol 12:672415 (2021). PubMed: 34093573
    • Schwabenland M  et al. Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions. Immunity 54:1594-1610.e11 (2021). PubMed: 34174183
    • Adorjan I  et al. Evidence for Decreased Density of Calretinin-Immunopositive Neurons in the Caudate Nucleus in Patients With Schizophrenia. Front Neuroanat 14:581685 (2020). PubMed: 33281566
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM119 antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6
    Permeabilization
    No
    Specification
    Prostate
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 21 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM119 antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ph6.0 Citrate Buffer
    Permeabilization
    Yes - Triton X-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Avinash Hari Narayanan

    Verified customer

    Submitted Aug 11 2017

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