Anti-TMEM120B antibody (ab121413)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TMEM120B antibody -
Description
Rabbit polyclonal to TMEM120B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen: THRIYKQKLE ELAALQTLCS SSISKQKKHL KDLKLTLQRC KRHASREEAE LVQQMAANIK ERQDVFFDME AYLPKKNGLY LNLVLGNVNV TLLSNQAKFA YKD, corresponding to amino acids 25-127 of Human TMEM120B (A0PK00).
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Positive control
- Human stomach tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| WB | 1/100 - 1/250. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the TMEM120 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 144404 Human
- SwissProt: A0PK00 Human
- Unigene: 644504 Human
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Alternative names
- T120B_HUMAN antibody
- TMEM120B antibody
- Transmembrane protein 120B antibody
Images
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Western blot - Anti-TMEM120B antibody (ab121413)Lane 1 : Anti-TMEM120B antibody (ab121413) at 1/100 dilution
Lane 2 : Anti-TMEM120B antibody (ab121413)
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag (~3.1kDa) in HEK293T cells.
Predicted band size: 40 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TMEM120B antibody (ab121413)Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli and cytoplasm. Recommended concentration of ab121413 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM120B antibody (ab121413)Immunohistochemical staining of Human stomach tissue shows strong cytoplasmic positivity in glandular cells, using 1/20 ab121413.
Protocols
Datasheets and documents
References
ab121413 has not yet been referenced specifically in any publications.
