Key features and details
- Rabbit polyclonal to TMEM120B
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TMEM120B antibody
DescriptionRabbit polyclonal to TMEM120B
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human stomach tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||1/100 - 1/250. Predicted molecular weight: 40 kDa.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the TMEM120 family.
- Information by UniProt
- T120B_HUMAN antibody
- TMEM120B antibody
- Transmembrane protein 120B antibody
Lane 1 : Anti-TMEM120B antibody (ab121413) at 1/100 dilution
Lane 2 : Anti-TMEM120B antibody (ab121413)
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag (~3.1kDa) in HEK293T cells.
Predicted band size: 40 kDa
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli and cytoplasm. Recommended concentration of ab121413 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical staining of Human stomach tissue shows strong cytoplasmic positivity in glandular cells, using 1/20 ab121413.
ab121413 has not yet been referenced specifically in any publications.