Key features and details
- Rabbit polyclonal to TMEM127
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TMEM127 antibody
DescriptionRabbit polyclonal to TMEM127
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan
Synthetic peptide corresponding to a region within amino acids 1-50 of Human TMEM127 (NP_060319.1)
- HeLa, 293T, Jurkat, NIH3T3 whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab123516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 26 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionControls cell proliferation acting as a negative regulator of TOR signaling pathway mediated by mTORC1. May act as a tumor suppressor.
Tissue specificityWidely expressed.
Involvement in diseaseDefects in TMEM127 are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
Sequence similaritiesBelongs to the TMEM127 family.
Cellular localizationCell membrane. Cytoplasm. Association of TMEM127 with the cell membrane is enhanced by inhibition of endocytosis. In the cytoplasm, it colocalizes with markers of early endosomal structures, Golgi apparatus and lysosomes.
- Information by UniProt
- FLJ20507 antibody
- FLJ22257 antibody
- TM127_HUMAN antibody
All lanes : Anti-TMEM127 antibody (ab123516) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 3 minutes
Detection of TMEM127 by Western Blot of Immunprecipitate.
Lane 1: ab123516 at 1µg/ml staining TMEM127 in HeLa whole cell lysate immunoprecipitated using ab123516 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
ab123516 has not yet been referenced specifically in any publications.