Anti-TMEM14B antibody (ab193198)
Key features and details
- Rabbit polyclonal to TMEM14B
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM14B antibody -
Description
Rabbit polyclonal to TMEM14B -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human TMEM14B aa 1 to the C-terminus.
Database link: Q9NUH8 -
Positive control
- WB: K562 whole cell lysate. IHC-P: Human kidney tissue. ICC: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
>95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
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ICC |
1/100.
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IHC-P |
1/100.
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Notes |
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WB
1/500 - 1/2000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). |
ICC
1/100. |
IHC-P
1/100. |
Target
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Sequence similarities
Belongs to the UPF0136 (TMEM14) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 81853 Human
- SwissProt: Q9NUH8 Human
- Unigene: 95600 Human
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Alternative names
- FLJ60468 antibody
- MGC1223 antibody
- OTTHUMP00000214289 antibody
see all
Images
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Immunocytochemistry analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling TMEM14B with ab193198 at a 1/100 dilution, followed by an Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG (H&L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling TMEM14B with ab193198 at a 1/100 dilution.
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Anti-TMEM14B antibody (ab193198) at 2 µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 12 kDa
Additional bands at: 13 kDa. We are unsure as to the identity of these extra bands.Western blot analysis of K562 cell lysate labeling TMEM14B with ab193198 at 2 ug/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab193198 has not yet been referenced specifically in any publications.