Anti-TMEM161A antibody (ab237533)
Key features and details
- Rabbit polyclonal to TMEM161A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM161A antibody
See all TMEM161A primary antibodies -
Description
Rabbit polyclonal to TMEM161A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human TMEM161A aa 29-98.
Database link: Q9NX61 -
Positive control
- WB: Rat liver tissue lysate. IHC-P: Human endometrial cancer tissue. ICC/IF: PC-3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 54, 42 kDa.
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IHC-P |
1/500 - 1/1000.
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ICC/IF |
1/50 - 1/500.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 54, 42 kDa. |
IHC-P
1/500 - 1/1000. |
ICC/IF
1/50 - 1/500. |
Target
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Function
May play a role in protection against oxidative stress. Overexpression leads to reduced levels of oxidant-induced DNA damage and apoptosis. -
Sequence similarities
Belongs to the TMEM161 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 54929 Human
- Entrez Gene: 364535 Rat
- SwissProt: Q9NX61 Human
- Unigene: 631629 Human
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Alternative names
- Adaptive response to oxidative stress protein 29 antibody
- AROS-29 antibody
- T161A_HUMAN antibody
see all
Images
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Anti-TMEM161A antibody (ab237533) at 1/500 dilution + Rat liver tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54, 42 kDa -
Paraffin-embedded human endometrial cancer tissue stained for TMEM161A with ab237533 at a 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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PC-3 (human prostate adenocarcinoma cell line) cells stained for TMEM161A (green) using ab237533 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237533 has not yet been referenced specifically in any publications.