Key features and details
- Rabbit polyclonal to TMEM161A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-TMEM161A antibody
See all TMEM161A primary antibodies
DescriptionRabbit polyclonal to TMEM161A
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human TMEM161A aa 29-98.
Database link: Q9NX61
- WB: Rat liver tissue lysate. IHC-P: Human endometrial cancer tissue. ICC/IF: PC-3 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%
Our Abpromise guarantee covers the use of ab237533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 54, 42 kDa.|
|IHC-P||1/500 - 1/1000.|
|ICC/IF||1/50 - 1/500.|
FunctionMay play a role in protection against oxidative stress. Overexpression leads to reduced levels of oxidant-induced DNA damage and apoptosis.
Sequence similaritiesBelongs to the TMEM161 family.
- Information by UniProt
- Adaptive response to oxidative stress protein 29 antibody
- AROS-29 antibody
- T161A_HUMAN antibody
Anti-TMEM161A antibody (ab237533) at 1/500 dilution + Rat liver tissue lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54, 42 kDa
Paraffin-embedded human endometrial cancer tissue stained for TMEM161A with ab237533 at a 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
PC-3 (human prostate adenocarcinoma cell line) cells stained for TMEM161A (green) using ab237533 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237533 has not yet been referenced specifically in any publications.