Overview

  • Product name

    Anti-TMEM161A antibody [EPR14369]
    See all TMEM161A primary antibodies
  • Description

    Rabbit monoclonal [EPR14369] to TMEM161A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TMEM161A aa 1-100. The exact sequence is proprietary.
    Database link: Q9NX61

  • Positive control

    • HT 1080, HepG2 and Human fetal liver lysates; Human lung adenocarcinoma tissue; Human infiltrating duct carcinoma of breast tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May play a role in protection against oxidative stress. Overexpression leads to reduced levels of oxidant-induced DNA damage and apoptosis.
  • Sequence similarities

    Belongs to the TMEM161 family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adaptive response to oxidative stress protein 29 antibody
    • AROS-29 antibody
    • T161A_HUMAN antibody
    • tmem161a antibody
    • Transmembrane protein 161A antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/100000 dilution

    Lane 1 : HT1080 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?

References

ab180954 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180954.
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