Recombinant
RabMAb

Anti-TMEM161B antibody [EPR14032] - N-terminal (ab184559)

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Overview

  • Product name
    Anti-TMEM161B antibody [EPR14032] - N-terminal
  • Description
    Rabbit monoclonal [EPR14032] to TMEM161B - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human TMEM161B aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: Q8NDZ6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • K562 cells, Human fetal kidney tissue lysate, HepG2 cell line lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14032
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40 - 1/60.
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Target

  • Relevance
    The exact functions of TMEM161B remain unknown. There are 3 isoforms produced by alternative splicing.
  • Cellular localization
    Membrane; Multi-pass membrane protein
  • Database links
    • Alternative names
      • FLB3342 antibody
      • MGC33214 antibody
      • OTTHUMP00000162000 antibody
      • PRO1313 antibody
      • Transmembrane protein 161B antibody
      see all

    Images

    • Immunoprecipitation - Anti-TMEM161B antibody [EPR14032] - N-terminal (ab184559)
      Immunoprecipitation - Anti-TMEM161B antibody [EPR14032] - N-terminal (ab184559)

      Western blot analysis of TMEM161B in immunoprecipitation pellets from K562 lysate ab184559 used at 1/50 dilution, Lane 1. Lane 2 shows PBS negative control. Secondary antibody, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at a 1/1500 dilution.

    • Western blot - Anti-TMEM161B antibody [EPR14032] - N-terminal (ab184559)
      Western blot - Anti-TMEM161B antibody [EPR14032] - N-terminal (ab184559)
      All lanes : Anti-TMEM161B antibody [EPR14032] - N-terminal (ab184559) at 1/5000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : K562 cell line lysate
      Lane 3 : HepG2 cell line lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

      Predicted band size: 55 kDa

    References

    ab184559 has not yet been referenced specifically in any publications.

    Publishing research using ab184559? Please let us know so that we can cite the reference in this datasheet.

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