Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14032] to TMEM161B - N-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
Product nameAnti-TMEM161B antibody [EPR14032] - N-terminal
See all TMEM161B primary antibodies
DescriptionRabbit monoclonal [EPR14032] to TMEM161B - N-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human TMEM161B aa 1-150 (N terminal). The exact sequence is proprietary.
Database link: Q8NDZ6
- K562 cells, Human fetal kidney tissue lysate, HepG2 cell line lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab184559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/40 - 1/60.|
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
RelevanceThe exact functions of TMEM161B remain unknown. There are 3 isoforms produced by alternative splicing.
Cellular localizationMembrane; Multi-pass membrane protein
- FLB3342 antibody
- MGC33214 antibody
- OTTHUMP00000162000 antibody
Western blot analysis of TMEM161B in immunoprecipitation pellets from K562 lysate ab184559 used at 1/50 dilution, Lane 1. Lane 2 shows PBS negative control. Secondary antibody, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at a 1/1500 dilution.
All lanes : Anti-TMEM161B antibody [EPR14032] - N-terminal (ab184559) at 1/5000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : K562 cell line lysate
Lane 3 : HepG2 cell line lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 55 kDa
ab184559 has not yet been referenced specifically in any publications.