Anti-TMEM164 antibody (ab122510)
Key features and details
- Rabbit polyclonal to TMEM164
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TMEM164 antibody -
Description
Rabbit polyclonal to TMEM164 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 1-38 (MSRYSYQSLL DWLYGGVDPS FAGNGGPDCA AFLSWQQR) of Human TMEM164.
-
Positive control
- Human lung tissue; RT4 and U251 MG cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH6.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
Notes |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH6. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
-
Sequence similarities
Belongs to the TMEM164 family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 84187 Human
- SwissProt: Q5U3C3 Human
- Unigene: 496572 Human
-
Alternative names
- AI316850 antibody
- AI852450 antibody
- AW547186 antibody
see all
Images
-
Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122510 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
ab122510 at 1/150 dilution staining TMEM164 in Paraffin-embedded Human Lung tissue by Immunohistochemistry.
-
All lanes : Anti-TMEM164 antibody (ab122510) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MG lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab122510 has not yet been referenced specifically in any publications.