Anti-TMEM16A antibody (ab53212)

ab53213 is the prediluted version of ab53212, otherwise they are the same product.

Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with samples from Zebrafish. All tested species covered by the 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

I have obtained the immungoen sequences for all our TMEM16A antibodies and checked the alignment of these with the 3 Zebrafish sequences you provided.

Regrettably, for most of these the alignments are too low for us to suggest they may detect in Zebrafish. However, there is one with reasonable alignment that you may like to consider trying. I can recmomend to review the online datasheet for further information.

ab84115
https://www.abcam.com/TMEM16A-antibody-ab84115.html (or use the following: https://www.abcam.com/TMEM16A-antibody-ab84115.html).

Immunogen: ################

Alignment with Zebrafish sequences provided:
Sequence 1: 68%
Sequence 2: 68%
Sequence 3: 72%

We recommend alignment should be over 85% to predict that an antibody will detect. It isstill possible that with 68-72% alignment therecould be some crossreactivity. However, even when alignmentis very highwe cannot guarantee this without further testing.


If you would like to test the ab84115 in Zebrafish, please contact me again by replying to this message prior to the purchase as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

Unfortunately we are not able to provide the blocking peptide for ab53212 at this time. I am very sorry that this product is not available, but if there is anything else that we can do for you please let me know and I'll be happy to help.

Thank you for your inquiry.

I can confirm that the IHC-Fr image was not generated by our lab but is taken from the following publication:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3013035/?tool=pubmed

Please follow the above link to find out more about the protocol they used.

I also would like to point out that this publication used aDylight 488 conjugated donkey anti rabbit antibody that was purchased from a competitor and I can therefore not comment on the dilution that would be suitable.

But I am happy to confirm that antibodies from our catalog are also Dylight 488 conjugated donkey anti rabbit antibodies and suitable for your customers purpose. They can be used at a dilution (of the stock in the delivered vial) of 1;50 to 1:500.

ab98488 and ab96919 weresuccessfully used in a 1:200 dilution. To review the images please also follow the link to the respective datasheets:

ab98488

https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab98488.html (or use the following: https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab98488.html).

ab96919

https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html).

I hope this information is helpful for your customer and wish them good luck with their research.

Thank you for contacting us.
This image is from publication; Dutta AK et al, J Biol Chem. 2011 Jan 7;286(1):766-76. Epub 2010 Nov 1, Fig 1. DOI 10.1074/jbc.M110.164970. Customer can ask this to Author of the paper.
We also have DAPI mounting media in catalogue. The catalogue numbers are ab104139, ab10140
https://www.abcam.com/Fluoroshield-Mounting-Medium-With-DAPI-20ml-ab104139.html
https://www.abcam.com/Fluoroshield-Mounting-Medium-With-DAPI-ab104140.html
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thanks for your reply.

To calculate the 89% score, did you use the canis lupus sequence that I sent or a different dog sequence? I ran the BLAST again and the Identity score came up as 94%, so I'm not sure if we're looking at the same canine sequence.

Another option would be ab84115, which gave an Identity score of 98% with the canis lupus sequence-

https://www.abcam.com/TMEM16A-antibody-ab84115.html

If you would like to try either of these antibodies in canine samples, please let me know because we do have a testing discount program that you will be eligible for. In this program, you would purchase the TMEM16A antibody, try it in canine samples, submit an Abreview with your data, and in exchange we will give you one free primary antibody on a future order. Please let me know if this is something you're interested in and I'll send more information.

Please let me know if you have any questions or if there is anything else that I can do for you.

Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Human breast cancer 2.Anti-Progesterone Receptor antibody [SP2] (ab16661) Human breast carcinoma 3.Anti-Estrogen Receptor antibody [SP1] (ab16660) Human breast carcinoma 4.Anti-Myeloperoxidase antibody (ab45977) Human spleen tissue 5.Anti-TdT antibody (ab85148) Human testis 6.Anti-TMEM16A antibody (ab53212) Human liver 7.Anti-NSE antibody [EPR3377] (ab79757) Human brain tissue 8.Anti-CD23 antibody [EPR3617(IHC)] (ab92495) Human tonsil 9.Anti-CD10 antibody [56C6] (ab951) Lymph node tissue or human breast microepithelium 10.Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) Murine melanoma metastasis tissue 11.Anti-CD79a antibody [HM47/A9] (ab3121) Human Lymphoid tissue, tonsil 12.Anti-CD45RO antibody [UCHL-1] (ab86080) Human Tonsil, Thymus 13.Anti-CD45 antibody (ab10559) Human Tonsil tissue 14.Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) Human Hodgekins lymphoma tissue 15.Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Human Hodgekins lymphoma tissue Human spleen I am providing a list ofslides that we sell which I hope will be helpful to you. I can recommend to review the online datasheet for more details. Human brain: ab4305Brain (human): hippocampus normal tissue slides https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html (or use the following: https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html). Human lymph node: ab4350Lymph node (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html (or use the following: https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html). Human testis: ab4373Testis (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html (or use the following: https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html). Human liver: ab4348Liver (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html (or use the following: https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html). Human spleen: ab4372Spleen (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html (or use the following: https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html). I am sorry we do not have slides forthe following: Human tonsil Hodgekins lymphoma tissue Mouse melenoma tissue I hope this will be helpful to you. If you have any further questions,please do not hesitate to contact us.