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    tmem16a-antibody-ab53212.pdf

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Anti-TMEM16A antibody (ab53212)

  • Datasheet
  • SDS
Reviews (4)Q&A (9)References (87)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody (ab53212)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody (ab53212)

Key features and details

  • Rabbit polyclonal to TMEM16A
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-TMEM16A antibody [EPR10643(2)] (ab190803)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-TMEM16A antibody
    See all TMEM16A primary antibodies
  • Description

    Rabbit polyclonal to TMEM16A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human TMEM16A aa 400-500. The exact sequence is proprietary.
    Database link: Q5XXA6

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/100.
Notes
IHC-P
1/100.

Target

  • Function

    Acts as a calcium-activated chloride channel. Required for normal tracheal development.
  • Tissue specificity

    Broadly expressed with higher levels in liver and skeletal muscle.
  • Sequence similarities

    Belongs to the anoctamin family.
  • Domain

    The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • Cellular localization

    Cell membrane. Cytoplasm.
  • Target information above from: UniProt accession Q5XXA6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55107 Human
    • Entrez Gene: 101772 Mouse
    • Entrez Gene: 309135 Rat
    • Omim: 610108 Human
    • SwissProt: Q5XXA6 Human
    • SwissProt: Q8BHY3 Mouse
    • Unigene: 503074 Human
    • Unigene: 26700 Mouse
    • Alternative names

      • ANO 1 antibody
      • ANO1 antibody
      • ANO1_HUMAN antibody
      • Anoctamin 1 antibody
      • Anoctamin 1 calcium activated chloride channel antibody
      • Anoctamin-1 antibody
      • Anoctamin1 antibody
      • Ca2+ activated Cl- channel antibody
      • Calcium Activated Chloride Channel antibody
      • Discovered on gastrointestinal stromal tumors protein 1 antibody
      • DOG 1 antibody
      • DOG1 antibody
      • FLJ10261 antibody
      • Membrane protein antibody
      • Oral cancer overexpressed 2 antibody
      • Oral cancer overexpressed protein 2 antibody
      • ORAOV 2 antibody
      • ORAOV2 antibody
      • TAOS 2 antibody
      • TAOS2 antibody
      • TMEM 16A antibody
      • TMEM16A antibody
      • Transmembrane protein 16A (eight membrane spanning domains) antibody
      • Transmembrane protein 16A antibody
      • Tumor amplified and overexpressed sequence 2 antibody
      • Tumor-amplified and overexpressed sequence 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody (ab53212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody (ab53212)
      ab53212 (2µg/ml) staining TMEM16A in human liver (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of hepatocyte cell membrane.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody (ab53212)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody (ab53212)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastrointestinal stromal tumor labeling TMEM16A with ab53212.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (87)

    Publishing research using ab53212? Please let us know so that we can cite the reference in this datasheet.

    ab53212 has been referenced in 87 publications.

    • Guarascio DM  et al. Functional expression of TMEM16A in taste bud cells. J Physiol 599:3697-3714 (2021). PubMed: 34089532
    • Suzuki T  et al. TMEM16A Ca2+-Activated Cl- Channel Regulates the Proliferation and Migration of Brain Capillary Endothelial Cells. Mol Pharmacol 98:61-71 (2020). PubMed: 32358165
    • Grainger N  et al. Identification and classification of interstitial cells in the mouse renal pelvis. J Physiol 598:3283-3307 (2020). PubMed: 32415739
    • Naik AS  et al. Smad4-dependent morphogenic signals control the maturation and axonal targeting of basal vomeronasal sensory neurons to the accessory olfactory bulb. Development 147:N/A (2020). PubMed: 32341026
    • Guo JW  et al. Hepatocyte TMEM16A Deletion Retards NAFLD Progression by Ameliorating Hepatic Glucose Metabolic Disorder. Adv Sci (Weinh) 7:1903657 (2020). PubMed: 32440483
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 13 Abreviews or Q&A

    Western blot abreview for Anti-TMEM16A antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (submandibular gland)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    submandibular gland
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 12 2020

    IHC - Wholemount abreview for Anti-TMEM16A antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Guinea pig Tissue (intestine myenteric plexus)
    Specification
    intestine myenteric plexus
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 29 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM16A antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 11 2016

    Question

    Ab53212 and Ab53213

    Ab53213 we used for western blotting and IHC on cells, and it works fine.

    My question is, what is the difference between the two antibodies? On the datasheets it’s the same test applications mentioned and also the species they both react with. Do they target the same chromosomal location?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 16 2013

    Answer

    ab53213 is the prediluted version of ab53212, otherwise they are the same product.

    Read More

    Abcam Scientific Support

    Answered on Jan 16 2013

    Question

    Attached please find the amino acid sequences of interest of zebrafish (a total of 3). Thanks in advance!

    Kind Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 20 2012

    Answer

    Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

    I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with samples from Zebrafish. All tested species covered by the 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

    I have obtained the immungoen sequences for all our TMEM16A antibodies and checked the alignment of these with the 3 Zebrafish sequences you provided.

    Regrettably, for most of these the alignments are too low for us to suggest they may detect in Zebrafish. However, there is one with reasonable alignment that you may like to consider trying. I can recmomend to review the online datasheet for further information.

    ab84115
    https://www.abcam.com/TMEM16A-antibody-ab84115.html (or use the following: https://www.abcam.com/TMEM16A-antibody-ab84115.html).

    Immunogen: ################

    Alignment with Zebrafish sequences provided:
    Sequence 1: 68%
    Sequence 2: 68%
    Sequence 3: 72%

    We recommend alignment should be over 85% to predict that an antibody will detect. It isstill possible that with 68-72% alignment therecould be some crossreactivity. However, even when alignmentis very highwe cannot guarantee this without further testing.


    If you would like to test the ab84115 in Zebrafish, please contact me again by replying to this message prior to the purchase as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More

    Abcam Scientific Support

    Answered on Aug 20 2012

    Question

    Is the blocking peptide available for this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 18 2012

    Answer

    Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

    Unfortunately we are not able to provide the blocking peptide for ab53212 at this time. I am very sorry that this product is not available, but if there is anything else that we can do for you please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Jun 18 2012

    Question

    Hi,



    I have a customer enquiry about the protocol used for TMEM16A antibody (ab53212). Specifically their enquiry is about the protocol used by a research group listed in the Anti-TMEM16A images- IHC Frozen sections.

    “On the information site for ab53212 it states under "Immunohistochemistry (Frozen sections) - TMEM16A antibody (ab53212) that "Sections were incubated with Dylight 488 conjugated donkey anti-rabbit secondary at a 1/600 dilution" The product comes as 0.5mg/ml. Is this a 1/600 dilution of the 0.5mg/ml stock? thank you”

    Can you please identify the particular secondary antibody that this group used, and confirm that the 1/600 dilution used by the group is a dilution of the stock solution.



    Thank you!



    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 14 2012

    Answer

    Thank you for your inquiry.

    I can confirm that the IHC-Fr image was not generated by our lab but is taken from the following publication:

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3013035/?tool=pubmed

    Please follow the above link to find out more about the protocol they used.

    I also would like to point out that this publication used aDylight 488 conjugated donkey anti rabbit antibody that was purchased from a competitor and I can therefore not comment on the dilution that would be suitable.

    But I am happy to confirm that antibodies from our catalog are also Dylight 488 conjugated donkey anti rabbit antibodies and suitable for your customers purpose. They can be used at a dilution (of the stock in the delivered vial) of 1;50 to 1:500.

    ab98488 and ab96919 weresuccessfully used in a 1:200 dilution. To review the images please also follow the link to the respective datasheets:

    ab98488

    https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab98488.html (or use the following: https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab98488.html).

    ab96919

    https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html).

    I hope this information is helpful for your customer and wish them good luck with their research.

    Read More

    Abcam Scientific Support

    Answered on Jun 14 2012

    Question

    On the Datasheet for Anti-TMEM16A antibody (ab53212), under the Immunohistochemistry (Frozen sections) its states:

    Sections were defrosted at room temperature and fixed in 4% paraformaldehyde (1× PBS), permeabilized in PBST (1× PBS with 0.2% Triton), and blocked in 4% BSA (in PBST) for 1 hour at room temperature. Samples were then incubated with ab53212 at a 1/100 dilution, diluted in 1% BSA. Sections were incubated overnight at 4 °C and washed three times for 10 minutes each with 1× PBST at room temperature. Sections were incubated with Dylight 488 conjugated donkey anti-rabbit secondary at a 1/600 dilution for 2 hours at room temperature protected from light. Following incubation, the slides were washed in PBST at room temperature and coverslipped with Antifade gold and DAPI. Images were visualized using an Olympus IX-71 confocal microscope.

    Our customer would like to find out cat# for Antifade gold and DAPI.

    Your help will greatly appreciated.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 13 2012

    Answer

    Thank you for contacting us.
    This image is from publication; Dutta AK et al, J Biol Chem. 2011 Jan 7;286(1):766-76. Epub 2010 Nov 1, Fig 1. DOI 10.1074/jbc.M110.164970. Customer can ask this to Author of the paper.
    We also have DAPI mounting media in catalogue. The catalogue numbers are ab104139, ab10140
    https://www.abcam.com/Fluoroshield-Mounting-Medium-With-DAPI-20ml-ab104139.html
    https://www.abcam.com/Fluoroshield-Mounting-Medium-With-DAPI-ab104140.html
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jun 13 2012

    Question

    Thank you,about the anti-TMEM16A one,the

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    Identities is only 89%,this is too low for us,do you have any recommendation for anti-TMEM16A that possibly works on dog

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2012

    Answer

    Thanks for your reply.

    To calculate the 89% score, did you use the canis lupus sequence that I sent or a different dog sequence? I ran the BLAST again and the Identity score came up as 94%, so I'm not sure if we're looking at the same canine sequence.

    Another option would be ab84115, which gave an Identity score of 98% with the canis lupus sequence-

    https://www.abcam.com/TMEM16A-antibody-ab84115.html

    If you would like to try either of these antibodies in canine samples, please let me know because we do have a testing discount program that you will be eligible for. In this program, you would purchase the TMEM16A antibody, try it in canine samples, submit an Abreview with your data, and in exchange we will give you one free primary antibody on a future order. Please let me know if this is something you're interested in and I'll send more information.

    Please let me know if you have any questions or if there is anything else that I can do for you.

    Read More

    Abcam Scientific Support

    Answered on Feb 28 2012

    Question

    Dear Sir/Madam Please kindly recommend the abcam slide control for IHC-P application for this antibodies. 1. Anti-ErbB 2 antibody (ab2428) 2. Anti-Progesterone Receptor antibody [SP2] (ab16661) 3. Anti-Estrogen Receptor antibody [SP1] (ab16660) 4. Anti-Myeloperoxidase antibody (ab45977) 5. Anti-TdT antibody (ab85148) 6. Anti-TMEM16A antibody (ab53212) 7. Anti-NSE antibody [EPR3377] (ab79757) 8. Anti-CD23 antibody [EPR3617(IHC)] (ab92495) 9. Anti-CD10 antibody [56C6] (ab951) 10. Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) 11. Anti-CD79a antibody [HM47/A9] (ab3121) 12. Anti-CD45RO antibody [UCHL-1] (ab86080) 13. Anti-CD45 antibody (ab10559) 14. Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) 15. Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jan 09 2012

    Answer

    Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Human breast cancer 2.Anti-Progesterone Receptor antibody [SP2] (ab16661) Human breast carcinoma 3.Anti-Estrogen Receptor antibody [SP1] (ab16660) Human breast carcinoma 4.Anti-Myeloperoxidase antibody (ab45977) Human spleen tissue 5.Anti-TdT antibody (ab85148) Human testis 6.Anti-TMEM16A antibody (ab53212) Human liver 7.Anti-NSE antibody [EPR3377] (ab79757) Human brain tissue 8.Anti-CD23 antibody [EPR3617(IHC)] (ab92495) Human tonsil 9.Anti-CD10 antibody [56C6] (ab951) Lymph node tissue or human breast microepithelium 10.Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) Murine melanoma metastasis tissue 11.Anti-CD79a antibody [HM47/A9] (ab3121) Human Lymphoid tissue, tonsil 12.Anti-CD45RO antibody [UCHL-1] (ab86080) Human Tonsil, Thymus 13.Anti-CD45 antibody (ab10559) Human Tonsil tissue 14.Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) Human Hodgekins lymphoma tissue 15.Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Human Hodgekins lymphoma tissue Human spleen I am providing a list ofslides that we sell which I hope will be helpful to you. I can recommend to review the online datasheet for more details. Human brain: ab4305Brain (human): hippocampus normal tissue slides https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html (or use the following: https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html). Human lymph node: ab4350Lymph node (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html (or use the following: https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html). Human testis: ab4373Testis (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html (or use the following: https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html). Human liver: ab4348Liver (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html (or use the following: https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html). Human spleen: ab4372Spleen (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html (or use the following: https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html). I am sorry we do not have slides forthe following: Human tonsil Hodgekins lymphoma tissue Mouse melenoma tissue I hope this will be helpful to you. If you have any further questions,please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Jan 09 2012

    1-10 of 13 Abreviews or Q&A

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