• Product name

  • Description

    Rabbit polyclonal to TMEM16A
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 828-877 (HGFVNHTLSS FNVSDFQNGT APNDPLDLGY EVQICRYKDY REPPWSENKY) of Human TMEM16A (NP_060513).

  • Positive control

    • Human fetal muscle lysate.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab84115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function

    Acts as a calcium-activated chloride channel. Required for normal tracheal development.
  • Tissue specificity

    Broadly expressed with higher levels in liver and skeletal muscle.
  • Sequence similarities

    Belongs to the anoctamin family.
  • Domain

    The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • Cellular localization

    Cell membrane. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • DOG1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all


  • Anti-TMEM16A antibody (ab84115) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 114 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

    Gel concentration: 6-18%


This product has been referenced in:

  • Dayal A  et al. Ca2+-activated Cl- channel TMEM16A/ANO1 identified in zebrafish skeletal muscle is crucial for action potential acceleration. Nat Commun 10:115 (2019). Read more (PubMed: 30631052) »
  • Crutzen R  et al. Anoctamin 1 (Ano1) is required for glucose-induced membrane potential oscillations and insulin secretion by murine ß-cells. Pflugers Arch 468:573-91 (2016). Read more (PubMed: 26582426) »
See all 3 Publications for this product

Customer reviews and Q&As


I have had the opportunity to review our anti-TMEM16A antibodies and have found two which will recognize the extracelluar domain of this target. https://www.abcam.com/tmem16a-antibody-ab84115.html was created from a peptide corresponding to a region within amino acids 828-877 (HGFVNHTLSS FNVSDFQNGT APNDPLDLGY EVQICRYKDY REPPWSENKY) of Human TMEM16A (NP_060513). Based on Uniprot information (Q5XXA6 ) this region is extracellular. https://www.abcam.com/tmem16a-antibody-sp31-ab64085.html was made using a cocktail of 3 different immunogens; two are from the cytoplasmic domain and one is from the extracellular domain.

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