Overview

  • Product name

    Anti-TMEM16A antibody - C-terminal
    See all TMEM16A primary antibodies
  • Description

    Rabbit polyclonal to TMEM16A - C-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TMEM16A aa 927-943 (C terminal).
    Sequence:

    KVLMVELFMREEQDKQQ


    Database link: Q5XXA6

  • Positive control

    • Rat liver and Rat skeletal muscle tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.025% Sodium azide
    Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2% Trehalose
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).

Target

  • Function

    Acts as a calcium-activated chloride channel. Required for normal tracheal development.
  • Tissue specificity

    Broadly expressed with higher levels in liver and skeletal muscle.
  • Sequence similarities

    Belongs to the anoctamin family.
  • Domain

    The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • Cellular localization

    Cell membrane. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • DOG1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all

Images

  • All lanes : Anti-TMEM16A antibody - C-terminal (ab191040) at 0.5 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 114 kDa
    Observed band size: 114 kDa

References

ab191040 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293 transfected with mouse TMEM16A)
Gel Running Conditions
Reduced Denaturing (8% Acrylamide Gel)
Loading amount
20 µg
Treatment
Transfected with mouse TMEM16A vector
Specification
HEK293 transfected with mouse TMEM16A
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 06 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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