Anti-TMEM16A antibody [DG1/447 + DOG-1.1] - BSA and Azide free (ab212745)

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Overview

  • Product name

    Anti-TMEM16A antibody [DG1/447 + DOG-1.1] - BSA and Azide free
    See all TMEM16A primary antibodies

  • Description

    Mouse monoclonal [DG1/447 + DOG-1.1] to TMEM16A - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Fr, IP, WB, ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Human TMEM16A aa 904-986 (C terminal). Immunogen used to raise the antibody in DOG-1.1. conjugated to a carrier protein.
    Sequence:

    MSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQDKQQLLETCMEKER QKDEPPCNHHNTKACPDSLGSPAPSHAYHGGVL


    Database link: Q5XXA6

    Recombinant full length protein corresponding to Human TMEM16A aa 1-986. Immunogen used to raise the antibody in DG1/447.
    Sequence: MRVNEKYSTLPAEDRSVHIINICAIEDIGYLPSEGTLLNSLSVDPDAECK YGLYFRDGRRKVDYILVYHHKRPSGNRTLVRRVQHSDTPSGARSVKQDHP LPGKGASLDAGSGEPPMDYHEDDKRFRREEYEGNLLEAGLELERDEDTKI HGVGFVKIHAPWNVLCREAEFLKLKMPTKKMYHINETRGLLKKINSVLQK ITDPIQPKVAEHRPQTMKRLSYPFSREKQHLFDLSDKDSFFDSKTRSTIV YEILKRTTCTKAKYSMGITSLLANGVYAAAYPLHDGDYNGENVEFNDRKL LYEEWARYGVFYKYQPIDLVRKYFGEKIGLYFAWLGVYTQMLIPASIVGI IVFLYGCATMDENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARA SHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAV KDHPRAEYEARVLEKSLKKESRNKEKRRHIPEESTNKWKQRVKTAMAGVK LTDKVKLTWRDRFPAYLTNLVSIIFMIAVTFAIVLGVIIYRISMAAALAM NSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEK SFEERLIFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEE CAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKLIRYLKLKQQSP PDHEECVKRKQRYEVDYNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAP LFALLNNIIEIRLDAKKFVTELRRPVAVRAKDIGIWYNILRGIGKLAVII NAFVISFTSDFIPRLVYLYMYSKNGTMHGFVNHTLSSFNVSDFQNGTAPN DPLDLGYEVQICRYKDYREPPWSENKYDISKDFWAVLAARLAFVIVFQNL VMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQDKQQLLETWME KERQKDEPPCNHHNTKACPDSLGSPAPSHAYHGGVL
    Database link: Q5XXA6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.

  • General notes

    ab212745 is a carrier free version of ab200486. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    DG1/447 + DOG-1.1

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab212745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
IP Use at 1-2 µg/mg of lysate.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 114 kDa.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 10 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a calcium-activated chloride channel. Required for normal tracheal development.

  • Tissue specificity

    Broadly expressed with higher levels in liver and skeletal muscle.

  • Sequence similarities

    Belongs to the anoctamin family.

  • Domain

    The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.

  • Cellular localization

    Cell membrane. Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANO 1 antibody
      • ANO1 antibody
      • ANO1_HUMAN antibody
      • Anoctamin 1 antibody
      • Anoctamin 1 calcium activated chloride channel antibody
      • Anoctamin-1 antibody
      • Anoctamin1 antibody
      • Ca2+ activated Cl- channel antibody
      • Calcium Activated Chloride Channel antibody
      • Discovered on gastrointestinal stromal tumors protein 1 antibody
      • DOG 1 antibody
      • DOG1 antibody
      • FLJ10261 antibody
      • Membrane protein antibody
      • Oral cancer overexpressed 2 antibody
      • Oral cancer overexpressed protein 2 antibody
      • ORAOV 2 antibody
      • ORAOV2 antibody
      • TAOS 2 antibody
      • TAOS2 antibody
      • TMEM 16A antibody
      • TMEM16A antibody
      • Transmembrane protein 16A (eight membrane spanning domains) antibody
      • Transmembrane protein 16A antibody
      • Tumor amplified and overexpressed sequence 2 antibody
      • Tumor-amplified and overexpressed sequence 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody (ab212745)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody (ab212745)

      Immunohistochemical analysis of paraffin-embedded Human gastrointestinal stromal tumor tissue, labeling TMEM16A using ab200486 at 1 μg/ml. Zoom 20X.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200486)

      .

    References

    ab212745 has not yet been referenced specifically in any publications.

    Publishing research using ab212745? Please let us know so that we can cite the reference in this datasheet.

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