Recombinant Anti-TMEM16A antibody [EPR10643(2)] (ab190803)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10643(2)] to TMEM16A
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TMEM16A antibody [EPR10643(2)]
See all TMEM16A primary antibodies -
Description
Rabbit monoclonal [EPR10643(2)] to TMEM16A -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human gastrointestinal stromal tumor tissue; K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10643(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Acts as a calcium-activated chloride channel. Required for normal tracheal development. -
Tissue specificity
Broadly expressed with higher levels in liver and skeletal muscle. -
Sequence similarities
Belongs to the anoctamin family. -
Domain
The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region. -
Cellular localization
Cell membrane. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55107 Human
- Omim: 610108 Human
- SwissProt: Q5XXA6 Human
- Unigene: 503074 Human
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Alternative names
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded Human gastrointestinal stromal tumor tissue sections labeling TMEM16A using ab190803 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of K562 cells labeling TMEM16A using ab190803 at a 1/70 dilution (pink). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control: Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab190803 has been referenced in 1 publication.
- Rodriguez TC et al. Reduced Expression of TMEM16A Impairs Nitric Oxide-Dependent Cl- Transport in Retinal Amacrine Cells. Front Cell Neurosci 16:937060 (2022). PubMed: 35966201