Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10643(2)] to TMEM16A
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
Product nameAnti-TMEM16A antibody [EPR10643(2)]
See all TMEM16A primary antibodies
DescriptionRabbit monoclonal [EPR10643(2)] to TMEM16A
Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TMEM16A aa 250-350. The exact sequence is proprietary.
Database link: Q5XXA6
- Human gastrointestinal stromal tumor tissue; K562 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab190803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionActs as a calcium-activated chloride channel. Required for normal tracheal development.
Tissue specificityBroadly expressed with higher levels in liver and skeletal muscle.
Sequence similaritiesBelongs to the anoctamin family.
DomainThe region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
Cellular localizationCell membrane. Cytoplasm.
- Information by UniProt
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
Immunohistochemical analysis of paraffin embedded Human gastrointestinal stromal tumor tissue sections labeling TMEM16A using ab190803 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometric analysis of K562 cells labeling TMEM16A using ab190803 at a 1/70 dilution (pink). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control: Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
ab190803 has not yet been referenced specifically in any publications.