Overview

  • Product name
    Anti-TMEM16A antibody, prediluted
    See all TMEM16A primary antibodies
  • Description
    Rabbit polyclonal to TMEM16A, prediluted
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Positive control
    • GIST

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, 0.605% Tris
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1.
IHC-Fr Use at an assay dependent concentration. PubMed: 20686072
ICC/IF Use at an assay dependent concentration. PubMed: 20686072

Target

  • Function
    Acts as a calcium-activated chloride channel. Required for normal tracheal development.
  • Tissue specificity
    Broadly expressed with higher levels in liver and skeletal muscle.
  • Sequence similarities
    Belongs to the anoctamin family.
  • Domain
    The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • Cellular localization
    Cell membrane. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • DOG1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all

Images

  • ab53213 staining TMEM16A in human gastrointestinal stromal tumor sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate pH 6.0. Samples were then incubated with undiluted ab53213 for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.

    See Abreview

References

This product has been referenced in:
  • Sonneville F  et al. MicroRNA-9 downregulates the ANO1 chloride channel and contributes to cystic fibrosis lung pathology. Nat Commun 8:710 (2017). Read more (PubMed: 28955034) »
  • Suzuki A  et al. Regulation of transient receptor potential vanilloid 1 expression in trigeminal ganglion neurons via methyl-CpG binding protein 2 signaling contributes tongue heat sensitivity and inflammatory hyperalgesia in mice. Mol Pain 12:N/A (2016). IHC . Read more (PubMed: 27030715) »
See all 14 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

1. The concentration is 1-2 ug/ml..

2. A green die is added to the buffer of this product. This color will not generate background or fluorescence.

Read More

Answer

Thank you very much for your reply and for keeping me update.

I'm sorry to hear that this antibody still did not work after modifying the protocol. I'd be happy to send a replacement with a different antibody or issue a credit or refund. Please let me know which option you'd like to accept and send me your original PO or order number, and I'll arrange it for you promptly.

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

Read More

Answer

Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with samples from Zebrafish. All tested species covered by the 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

I have obtained the immungoen sequences for all our TMEM16A antibodies and checked the alignment of these with the 3 Zebrafish sequences you provided.

Regrettably, for most of these the alignments are too low for us to suggest they may detect in Zebrafish. However, there is one with reasonable alignment that you may like to consider trying. I can recmomend to review the online datasheet for further information.

ab84115
https://www.abcam.com/index.html?datasheet=84115 (or use the following: https://www.abcam.com/index.html?datasheet=84115).

Immunogen: ################

Alignment with Zebrafish sequences provided:
Sequence 1: 68%
Sequence 2: 68%
Sequence 3: 72%

We recommend alignment should be over 85% to predict that an antibody will detect. It isstill possible that with 68-72% alignment therecould be some crossreactivity. However, even when alignmentis very highwe cannot guarantee this without further testing.


If you would like to test the ab84115 in Zebrafish, please contact me again by replying to this message prior to the purchase as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More

Answer

Je suis désolée d'apprendre que vous avez eu des problèmes avec le lot GR27833-1 ainsi que avec le Western blot sur tissu/cellules de souris.
Si vous souhaitez que nous regardons et investiguons ce problème ensemble, veuillez s'il vous plaît nous transmettre vos conditions expérimentales. Veuillez trouver ci-joint le questionnaire qui nous permet de regrouper le plus d'informations possible sur le protocole que vous avez utilisé avec cet anticorps.
Pour les résultats Western blot, j'ai juste trouvé ces images qui sont en lien sur la fiche technique aussi:
Western blot sur du tissue du souris:
https://www.abcam.com/index.html?datasheet=53213&tab=abreviews&intabreviewid=19768
Western blot sur du tissue du souris, bandes coupées:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2904657/?tool=pubmed
Western blot sur du tissue de souris ainsi que description de splice variants dans des différents tissues.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2980309/?tool=pubmed
Effectivement, aussi dans UniProt, trois différents isoformes de TMEM16A sont décrits . Les formes sont à 74kD, 98kD ainsi que 114kD.
http://www.uniprot.org/uniprot/Q5XXA6
Comme TMEM16A est une protéine avec plusieurs parties transmembranaires, nous recommandons de chauffer les lysats que à 70°C pour la dénaturation avant mis sur le gel, car autrement il y peut avoir dégradation.
J'espère que ces informations vous sont utiles. N'hésitez pas à nous recontacter, si vous souhaitez discuter les résultats en plus de détails ou si vous avez des autres questions.
Comme aussi discuté au téléphone, nous n'avons malheureusement pas les mêmes lots en stock. Je tiens à vous rassurer, que nous garantissons tous nos produits de fonctionner comme décrit sur la fiche technique. Voici le lien pour plus d'information sur notre garantie: www.abcam.com/abpromise
Je vous souhaite une très belle journée.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (GIST)
Specification
GIST
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6, 20 min, 100C
Permeabilization
No

Abcam user community

Verified customer

Submitted Dec 14 2011

Question
Answer

Thank you for contacting us. The immunogen is from amino acid range between 100 - 450 of human TMEM16A. I am sorry we do not have any additional immunogen information; this is the only information we have. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain and Lung)
Loading amount
20 µg
Specification
Brain and Lung
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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