Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 0.605% Tris
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab53213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20686072|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20686072|
FunctionActs as a calcium-activated chloride channel. Required for normal tracheal development.
Tissue specificityBroadly expressed with higher levels in liver and skeletal muscle.
Sequence similaritiesBelongs to the anoctamin family.
DomainThe region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
Cellular localizationCell membrane. Cytoplasm.
- Information by UniProt
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
ab53213 staining TMEM16A in human gastrointestinal stromal tumor sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate pH 6.0. Samples were then incubated with undiluted ab53213 for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.
This product has been referenced in:
- Sonneville F et al. MicroRNA-9 downregulates the ANO1 chloride channel and contributes to cystic fibrosis lung pathology. Nat Commun 8:710 (2017). Read more (PubMed: 28955034) »
- Suzuki A et al. Regulation of transient receptor potential vanilloid 1 expression in trigeminal ganglion neurons via methyl-CpG binding protein 2 signaling contributes tongue heat sensitivity and inflammatory hyperalgesia in mice. Mol Pain 12:N/A (2016). IHC . Read more (PubMed: 27030715) »