• Product name
    Anti-TMEM16A antibody [SP31]
    See all TMEM16A primary antibodies
  • Description
    Rabbit monoclonal [SP31] to TMEM16A
  • Host species
  • Tested applications
    Suitable for: IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human, Cynomolgus monkey
  • Positive control
    • Human GIST tumor


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA, Tris buffered saline
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab64085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC Use at an assay dependent concentration. PubMed: 23866066


  • Function
    Acts as a calcium-activated chloride channel. Required for normal tracheal development.
  • Tissue specificity
    Broadly expressed with higher levels in liver and skeletal muscle.
  • Sequence similarities
    Belongs to the anoctamin family.
  • Domain
    The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • Cellular localization
    Cell membrane. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • DOG1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all


  • Immunohistochemical analysis of Human Gastrointestinal Stromal Tumor tissue labelling TMEM16A with ab64085.


This product has been referenced in:
  • Nizyaeva NV  et al. Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia. Sci Rep 8:3453 (2018). IHC-P ; Human . Read more (PubMed: 29472628) »
  • Seo Y  et al. Inhibition of ANO1 by luteolin and its cytotoxicity in human prostate cancer PC-3 cells. PLoS One 12:e0174935 (2017). Read more (PubMed: 28362855) »

See all 25 Publications for this product

Customer reviews and Q&As

I have had the opportunity to review our anti-TMEM16A antibodies and have found two which will recognize the extracelluar domain of this target. https://www.abcam.com/tmem16a-antibody-ab84115.html was created from a peptide corresponding to a regio...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with sam...

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Thank you for your phone call.

Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab64085 in ICC/IF. This code will give you: 1 free PRIMARY ANTIBODY before the expira...

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Thank you for contacting Abcam regarding ab64085.

The immunogen for this antibody is not located within aa 650-706 thus it should not be affected by the paper's findings (Circ Res March 30 2012. Vol 110, p990-999 ).

Please do...

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ab64085 was made using a cocktail of 3 different immunogens; one is from the pore-forming region and the other two are not.  Unfortunately the epitope for this antibody has not been mapped, so we do not know where the target is.  Please do not ...

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Unfortunately the exact immunogen sequence of this antibody is proprietary. I can tell you that the Anti-TMEM16A antibody [SP31] (ab64085) which you are interested is produced using a cocktail of 3 peptides, two of which are located in cytoplasmic and ...

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Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Rat Tissue lysate - whole (Whole brain)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
40 µg
1X SDS for 10 mins
Whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Submitted Nov 06 2009


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